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STRAIGHT-IN Dual: a platform for dual, single-copy integrations of DNA payloads and gene circuits into human induced pluripotent stem cells.

Blanch-Asensio A, Ploessl DS, Wang NB, Mummery CL, Galloway KE, Davis RP
bioRxiv [Preprint]. 2024 Oct 17:2024.10.17.616637. doi: 10.1101/2024.10.17.616637. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
229776CLYBL_Bxb1-GA_LP_Dual_TCCLYBL targeting construct with the Bxb1-GA landing pad cassette.
229777Bxb1-GT_CAG_3xTurboGFP-NLSBxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of nuclear-localised TurboGFP (3 copies)
229778Bxb1-GT_CAG-ITPKA-mScarlet-IBxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of a mScarlet-I F-actin reporter
229779Bxb1-GA Donor_hEF1aBxb1-GA donor plasmid for the cloning of payloads <20 kb with hEF1a promoter for expressing landing pad enrichment marker
229780Bxb1-GA Donor_CAGBxb1-GA donor plasmid for the cloning of payloads <20 kb with CAG promoter for expressing landing pad enrichment marker
229781Bxb1-GA_CAG-TurboGFPBxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of nuclear-localised TurboGFP
229782Bxb1-GA_CAG-ITPKA-3x-mScarlet-IBxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of a mScarlet-I F-actin reporter (3 copies)
229783Bxb1-GT_AIO-TetOn_Down-Tandem DonorBxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression (empty)
229784Bxb1-GT_AIO-TetOn_Divergent DonorBxb1-GT donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)
229785Bxb1-GA_AIO-TetOn_Down-Tandem DonorBxb1-GA donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression (empty)
229786Bxb1-GA_AIO-TetOn_Divergent DonorBxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)
229787Bxb1-GA_AIO-GZV-SynZiFTR_Down-Tandem donorBxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression (empty)
229788Bxb1-GA_AIO-GZV-SynZiFTR_mGL donorBxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible mGreenLantern expression
229789Bxb1-GA_AIO-GZV-SynZiFTR_mNgn2 donorBxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression of mouse neurogenin 2
229790Bxb1-GA_AIO-TetOn_mScarlet_ConvergentBxb1-GA donor plasmid with convergent syntax for all-in-one doxycycline-inducible mScarlet expression
229791Bxb1-GA_AIO-TetOn_mScarlet_DivergentBxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible mScarlet expression
229792Bxb1-GA_AIO-TetOn_mScarlet_Down-TandemBxb1-GA donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible mScarlet expression
229793Bxb1-GA_AIO-TetOn_mScarlet_Up-TandemBxb1-GA donor plasmid with upstream tandem syntax for all-in-one doxycycline-inducible mScarlet expression
229794Bxb1-GT_AIO-TetOn_mScarlet_Down-TandemBxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible mScarlet expression
229795Bxb1-GT_AIO-TetOn_mNgn2 _Down-TandemBxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of mouse neurogenin 2
229796Bxb1-GT_TRE-mScarletBxb1-GT donor plasmid for doxycycline-inducible mScarlet expression
229797Bxb1-GA_CAG-rtTA-T2A-mTagBFP2Bxb1-GA donor plasmid for constitutive expression of reverse tetracycline transactivator (rtTA) and nuclear mTagBFP2
229798Bxb1-GA_TRE-jRCaMP1bBxb1-GA donor plasmid for doxycycline-inducible expression of jRCaMP1b calcium reporter.
229799Bxb1-GA_CAG-EGFPBxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of EGFP
229800Bxb1-GT_hEF1a-mGL_CAG-mRuby2_DivergentBxb1-GT donor plasmid with divergent syntax for constitutively expressing mRuby2 and mGreenLantern by the respective promoters CAG and hEF1a
229801Bxb1-GT_CAG-mRuby2Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mRuby2
229802Bxb1-GA_CAG-mStayGoldBxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold
232746pIVT-Bxb1IVT template for making modRNA encoding Bxb1
232747pIVT-evoBxb1IVT template for making modRNA encoding evoBxb1
232748pIVT-eeBxb1IVT template for making modRNA encoding eeBxb1
232749pIVT-Bxb1-I87LIVT template for making modRNA encoding mutant Bxb1 modRNA (I87L)
232750pIVT-Bxb1-I87L, A369P, E434GIVT template for making modRNA encoding mutant Bxb1modRNA (I87L, A369P, E434G)
232751pIVT-p53DDIVT template for making modRNA encoding p53DD, a mutant p53 that lacks DNA binding ability
232752pIVT-Flp-2A-BleoRIVT template for making bicistronic modRNA encoding Flippase and Zeocin resistance gene
232753pIVT-PuroRIVT template for making modRNA encoding the Puromycin resistance gene
232768pIVT-mGLIVT template for making modRNA encoding mGreenLantern
232773pIVT-iCreIVT template for making modRNA encoding iCre

Antibodies from Article