STRAIGHT-IN Dual: a platform for dual, single-copy integrations of DNA payloads and gene circuits into human induced pluripotent stem cells.

Blanch-Asensio A, Ploessl DS, Wang NB, Mummery CL, Galloway KE, Davis RP
bioRxiv [Preprint]. 2024 Oct 17:2024.10.17.616637. doi: 10.1101/2024.10.17.616637. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
229776	CLYBL_Bxb1-GA_LP_Dual_TC	CLYBL targeting construct with the Bxb1-GA landing pad cassette.
229779	Bxb1-GA Donor_hEF1a	Bxb1-GA donor plasmid for the cloning of payloads <20 kb with hEF1a promoter for expressing landing pad enrichment marker
229780	Bxb1-GA Donor_CAG	Bxb1-GA donor plasmid for the cloning of payloads <20 kb with CAG promoter for expressing landing pad enrichment marker
229782	Bxb1-GA_CAG-ITPKA-3x-mScarlet-I	Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of a mScarlet-I F-actin reporter (3 copies)
229783	Bxb1-GT_AIO-TetOn_Down-Tandem Donor	Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression (empty)
229784	Bxb1-GT_AIO-TetOn_Divergent Donor	Bxb1-GT donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)
229785	Bxb1-GA_AIO-TetOn_Down-Tandem Donor	Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression (empty)
229786	Bxb1-GA_AIO-TetOn_Divergent Donor	Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)
229787	Bxb1-GA_AIO-GZV-SynZiFTR_Down-Tandem donor	Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression (empty)
229788	Bxb1-GA_AIO-GZV-SynZiFTR_mGL donor	Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible mGreenLantern expression
229789	Bxb1-GA_AIO-GZV-SynZiFTR_mNgn2 donor	Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression of mouse neurogenin 2
229790	Bxb1-GA_AIO-TetOn_mScarlet_Convergent	Bxb1-GA donor plasmid with convergent syntax for all-in-one doxycycline-inducible mScarlet expression
229791	Bxb1-GA_AIO-TetOn_mScarlet_Divergent	Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible mScarlet expression
229792	Bxb1-GA_AIO-TetOn_mScarlet_Down-Tandem	Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible mScarlet expression
229793	Bxb1-GA_AIO-TetOn_mScarlet_Up-Tandem	Bxb1-GA donor plasmid with upstream tandem syntax for all-in-one doxycycline-inducible mScarlet expression
229794	Bxb1-GT_AIO-TetOn_mScarlet_Down-Tandem	Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible mScarlet expression
229795	Bxb1-GT_AIO-TetOn_mNgn2 _Down-Tandem	Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of mouse neurogenin 2
229796	Bxb1-GT_TRE-mScarlet	Bxb1-GT donor plasmid for doxycycline-inducible mScarlet expression
229797	Bxb1-GA_CAG-rtTA-T2A-mTagBFP2	Bxb1-GA donor plasmid for constitutive expression of reverse tetracycline transactivator (rtTA) and nuclear mTagBFP2
229798	Bxb1-GA_TRE-jRCaMP1b	Bxb1-GA donor plasmid for doxycycline-inducible expression of jRCaMP1b calcium reporter.
229799	Bxb1-GA_CAG-EGFP	Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of EGFP
229800	Bxb1-GT_hEF1a-mGL_CAG-mRuby2_Divergent	Bxb1-GT donor plasmid with divergent syntax for constitutively expressing mRuby2 and mGreenLantern by the respective promoters CAG and hEF1a
229801	Bxb1-GT_CAG-mRuby2	Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mRuby2
229802	Bxb1-GA_CAG-mStayGold	Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold