A Split-Luciferase-Based Trimer Formation Assay as a High-throughput Screening Platform for Therapeutics in Alport Syndrome.
Omachi K, Kamura M, Teramoto K, Kojima H, Yokota T, Kaseda S, Kuwazuru J, Fukuda R, Koyama K, Matsuyama S, Motomura K, Shuto T, Suico MA, Kai H
Cell Chem Biol. 2018 May 17;25(5):634-643.e4. doi: 10.1016/j.chembiol.2018.02.003. Epub 2018 Mar 8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 229768 | pFN33K LgBiT TK-neo Human collagen type IV alpha 5 chain (COL4A5) | Human Col4A5 tagged at N-terminal with LgBiT to be used with N-tagged SmBiT hCol4A3 and Flag-tagged hCol4A4 in Nanobit assay system |
| 229769 | pFC36K SmBiT TK-neo Human collagen type IV alpha 3 chain (COL4A3) | Human Col4A3 tagged at C-terminal with SmBiT to be used with C-tagged LgBiT hCol4A5 and Flag-tagged hCol4A4 in Nanobit assay system |
| 229770 | pFC34K LgBiT TK-Neo Human collagen type IV alpha 5 chain (COL4A5) | Human Col4A5 tagged at C-terminal with LgBiT to be used with C-tagged SmBiT hCol4A3 and Flag-tagged hCol4A4 in Nanobit assay system |