An E-cadherin-actin clutch translates the mechanical force of cortical flow for cell-cell contact to inhibit epithelial cell locomotion.
Noordstra I, Hermoso MD, Schimmel L, Bonfim-Melo A, Currin-Ross D, Duong CN, Kalappurakkal JM, Morris RG, Vestweber D, Mayor S, Gordon E, Roca-Cusachs P, Yap AS
Dev Cell. 2023 Sep 25;58(18):1748-1763.e6. doi: 10.1016/j.devcel.2023.06.011. Epub 2023 Jul 21.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 229696 | GFP-α-cateninV- | Transient expression of GFP-alpha-cateninV- in mammalian cells |
| 229697 | GFP-α-cateninA+ | Transient expression of GFP-alpha-cateninA+ in mammalian cells; enhanced actin binding |
| 229698 | GFP-α-cateninA- | Transient expression of GFP-alpha-cateninA- in mammalian cells |
| 229699 | pLViP-LifeAct-iRFP670 | Lentiviral expression of LifeAct-iRFP670 in mammalian cells |
| 229700 | pLViP-LifeAct-TagRFPT | Lentiviral expression of LifeAct-TagRFPT in mammalian cells |
| 229702 | pLViN-iRFP670-α-cateninWT | Lentiviral expression of iRFP670-alpha-cateninWT in mammalian cells |
| 229703 | pLViN-iRFP670-α-cateninA+ | Lentiviral expression of iRFP670-alpha-cateninA+ in mammalian cells |
| 229704 | GFP-α-cateninA+ΔβH | Transient expression of GFP-alpha-cateninA+deltabetaH in mammalian cells |
| 229705 | GFP-α-cateninΔβH | Transient expression of GFP-alpha-catenin-delta-beta-H in mammalian cells |
| 229706 | pLViN-iRFP670-α-cateninΔβH | Lentiviral expression of iRFP670-alpha-catenin-delta-beta-H in mammalian cells |
| 229707 | pLViN-iRFP670-α-cateninA+ΔβH | Lentiviral expression of iRFP670-alpha-cateninA+delta-beta-H in mammalian cells |
| 229708 | PX459-CTNNA1(α-catenin CRISPR KO) | Knockout of a-catenin in mammalian cells using CRISPR-Cas9 technology |
| 229709 | GFP-α-cateninWT | Transient expression of GFP-alpha-cateninWT in mammalian cells |
| 229710 | pLViN-E-cadherin-GFP | Lentiviral expression of E-cadherin-GFPTG in mammalian cells |