ARF1 compartments direct cargo flow via maturation into recycling endosomes.
Stockhammer A, Adarska P, Natalia V, Heuhsen A, Klemt A, Bregu G, Harel S, Rodilla-Ramirez C, Spalt C, Ozsoy E, Leupold P, Grindel A, Fox E, Mejedo JO, Zehtabian A, Ewers H, Puchkov D, Haucke V, Bottanelli F
Nat Cell Biol. 2024 Oct 4. doi: 10.1038/s41556-024-01518-4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 229676 | loxP-G418-loxP-3xALFA-Halo-Rab11 HR | Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with Halotag and 3x ALFA tag. Contains a G418 resistance cassette for selection of edited cells |
| 229677 | loxP-Puro-loxP-3xV5-SNAP-Rab11 HR | Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with SNAP tag and a 3x V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells. |
| 229678 | loxP-Hygro-loxP-HA-mStayGold-Rab11 HR | Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with monomeric StayGold and an HA epitope tag. Contains a hygromycin resistance cassette for selection of edited cells |
| 229679 | AP1muA-mStayGold-polyA-Hygro HR | Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with monomeric StayGold. Contains a hygromycin resistance cassette for selection of edited cells |
| 229680 | AP1muA-SNAP-V5-polyA-Puro HR | Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with SNAP tag and a V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells. |
| 229681 | AP1muA-Halo-ALFA-polyA-G418 HR | Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with HaloTag and an ALFA tag. Contains a G418 resistance cassette for selection of edited cells. |
| 229683 | Rab11 single guide RNA | guide RNA for Rab11 tagging at the N-terminus in pX459 |