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Hiten Madhani Lab: C. neoformans AID2 degron system plasmids

Unpublished

Plasmids from Article

ID Plasmid Purpose
229525pBHM2641Carries OsTIR1-t2a-mNG-AID*, used to insert TIR1 expressing construct into the C. neoformans genome.
229526pBHM2642Carries OsTIR1-t2a-mNG-mIAA7_opt, used to insert TIR1 expressing construct into the C. neoformans genome.
229527pBHM2643Carries OsTIR1-t2a-mNG-mAID_opt, used to insert TIR1 expressing construct into the C. neoformans genome.
229528pBHM2644Carries AID* tag, empty sgRNA, and NATR marker
229529pBHM2645Carries mIAA7_opt tag, empty sgRNA, and NATR marker
229530pBHM2646Carries mAID_opt tag, empty sgRNA, and NATR marker
229989pBHM2647Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.
229990pBHM2648Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.
229991pBHM2649Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.
229992pBHM2650Carries AID* tag, empty sgRNA, and HYGR marker
229993pBHM2651Carries AID* tag, empty sgRNA, and NEOR marker
229994pBHM2652Carries AID* tag, empty sgRNA, and amdS marker

Antibodies from Article