Functional screen identifies RBM42 as a mediator of oncogenic mRNA translation specificity.
Kovalski JR, Sarioglu G, Subramanyam V, Hernandez G, Rademaker G, Oses-Prieto JA, Slota M, Mohan N, Yiakis K, Liu I, Wen KW, Kim GE, Miglani S, Burlingame AL, Goodarzi H, Perera RM, Ruggero D
Nat Cell Biol. 2025 Feb 4. doi: 10.1038/s41556-024-01604-7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 229498 | Bidirectional MYC 5'UTR Dual Fluorescence Reporter | This is a lentiviral reporter for translation initiation under the control of the Myc 5'UTR, which produces destabilized GFP (dsGFP). There is a control destabilized mCherry (dsmCherry) as a control. |
| 229499 | pLEX Myc-HA | For lentiviral expression of Myc coding sequence with an HA tag. |
| 229500 | pLEX RBM42-HA | Lentiviral expression vector to express RBM42 isoform 1 with HA-tag. |
| 229501 | pLEX GFP-HA | Control HA-tagged protein for immunoprecipitation experiments. |
| 229502 | pGL3_5UTR_Myc_FLuc | Myc 5' untranslated region firefly luciferase reporter. |
| 229503 | pGL3_5UTR_Myc_FLuc RBM42 deletion | Myc 5' untranslated region firefly luciferase reporter with deletion of region bound by RBM42. |
| 229504 | pGL3_5UTR_Myc_FLuc Compensatory Mutant | Myc 5' untranslated region firefly luciferase reporter with compensatory mutations in the region bound by RBM42 |