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Photoactivated SOPP3 enables APEX2-mediated proximity labeling with high spatio-temporal resolution in live cells.

Qu D, Li Y, Liu Q, Cao B, Cao M, Lin X, Shen C, Zou P, Zhou H, Zhang W, Pan W
Cell Res. 2025 Feb;35(2):149-152. doi: 10.1038/s41422-024-01061-9. Epub 2024 Dec 10. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
229071SOPP3-HA-nesExpresses SOPP3 in the cytosol of mammalian cells
229072Sec61β-Flag-APEX2-SOPP3Expresse APEX2-SOPP3 in ER in mammalian cell
229073OMM-Flag-APEX2-SOPP3Expresses APEX2-SOPP3 targeted to outer mitochondrial membrane
229074Flag-APEX2-SOPP3-3xNLSexpresses APEX2-SOPP3 in the mammalian nucleus
229075COX8mls-Flag-APEX2-SOPP3Expresses APEX2-SOPP3 in the mitochondrial matrix
229077APEX2-SOPP3-TMExpresses APEX2-SOPP3 in the cell surface

Antibodies from Article