Cytosolic CRISPR RNAs for efficient application of RNA-targeting CRISPR-Cas systems.
Cheng ECK, Lam JKC, Kwon SC
EMBO Rep. 2025 Feb 26. doi: 10.1038/s44319-025-00399-4.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 229046 | pCI5-CasRx-HA (pKW88) | Mammalian expression of CasRx-HA |
| 229047 | pCI5-CasRx-NLS-HA (pKW919) | Mammalian expression of CasRx-NLS-HA |
| 229048 | pCI5-CasRx-NES-HA (pKW387) | Mammalian expression of CasRx-NES-HA |
| 229049 | pU6-CasRx-non-targeting (pEZ18) | Cloning backbone for CasRx crRNA (U6 promoter) |
| 229050 | phU1-CasRx-non-targeting (pKW82) | Cloning backbone for CasRx crRNA (Human U1 promoter) |
| 229051 | pmU1-CasRx-non-targeting (pKW83) | Cloning backbone for CasRx crRNA (Mouse U1 promoter) |
| 229052 | pU6-PspCas13b-non-targeting (pJK5) | Cloning backbone for PspCas13b crRNA (U6 promoter) |
| 229053 | phU1-PspCas13b-non-targeting (pEZ36) | Cloning backbone for PspCas13b crRNA (Human U1 promoter) |
| 229054 | pU6-Cas13bt1-non-targeting (pJK2) | Cloning backbone for Cas13bt1 crRNA (U6 promoter) |
| 229055 | phU1-Cas13bt1-non-targeting (pEZ25) | Cloning backbone for Cas13bt1 crRNA (Human U1 promoter) |
| 229056 | pU6-RanCas13b-non-targeting (pJK6) | Cloning backbone for RanCas13b crRNA (U6 promoter) |
| 229057 | phU1-RanCas13b-non-targeting (pEZ45) | Cloning backbone for RanCas13b crRNA (Human U1 promoter) |
| 229058 | pmirGLO-mir1-8mer4x (pEZ184) | Dual-luciferase reporter containing 4 copies of the 8mer site of human miR-1 |
| 229059 | pmirGLO-mir302-8mer4x (pEZ185) | Dual-luciferase reporter containing 4 copies of the 8mer site of human miR-302a |