SKI complex loss renders 9p21.3-deleted or MSI-H cancers dependent on PELO.

Borck PC, Boyle I, Jankovic K, Bick N, Foster K, Lau AC, Parker-Burns LI, Lubicki DA, Li T, Borah AA, Lofaso NJ, Das Sharma S, Chan T, Kishen RV, Adeagbo A, Raghavan S, Aquilanti E, Prensner JR, Krill-Burger JM, Golub TR, Campbell CD, Dempster JM, Chan EM, Vazquez F
Nature. 2025 Feb 5. doi: 10.1038/s41586-024-08509-3. PubMed Article

Plasmids from Article

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ID	Plasmid	Purpose
229024	pRDA052H_sgFOCAD #2	Expression of a EnAsCas12a guide targeting FOCAD
229023	pRDA052H_sgCh2	Expression of a EnAsCas12a control guide that cuts an intergenic region on chromosome 2
229021	pRDA355_sgCiCh2-2	Expression of a CRISPRi doxycycline inducible control sgRNA that cuts an intergenic region on chromosome 2
229020	pRDA355_sgCiPELO #2	Expression of a CRISPRi doxycycline inducible guide targeting PELO
229018	pXPR_BRD051d_sgCiPELO #2	Expression of CRISPRi PELO sgRNA #2
229017	pXPR_BRD051d_sgCiPELO #1	Expression of CRISPRi PELO sgRNA #1
228940	pXPR_051d_sgCiChr2-2	Expression of a CRISPRi control sgRNA that cuts an intergenic region on chromosome 2
228938	pLX313_PELO_WT	Constitutive expression of wild-type human PELO
228937	pRDA_052_HygroR	Plasmid for cloning of custom gRNA using EnAsCas12a
228935	pRDA_174_neoR	Lentiviral expression of EnAsCas12a