SKI complex loss renders 9p21.3-deleted or MSI-H cancers dependent on PELO.
Borck PC, Boyle I, Jankovic K, Bick N, Foster K, Lau AC, Parker-Burns LI, Lubicki DA, Li T, Borah AA, Lofaso NJ, Das Sharma S, Chan T, Kishen RV, Adeagbo A, Raghavan S, Aquilanti E, Prensner JR, Krill-Burger JM, Golub TR, Campbell CD, Dempster JM, Chan EM, Vazquez F
Nature. 2025 Feb 5. doi: 10.1038/s41586-024-08509-3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 228932 | pXPR_023d sgCiPELO #1 | Expression of dCas9-KRAB and sgRNA targeting PELO |
| 228934 | pXPR_023d sgCiPELO #8 | Expression of dCas9-KRAB and sgRNA targeting PELO |
| 228935 | pRDA_174_neoR | Lentiviral expression of EnAsCas12a |
| 228936 | pXPR_051_dCas9-KRAB-HA | All-in-one dCas9-KRAB-MeCP2 plasmid for cloning of custom sgRNA |
| 228937 | pRDA_052_HygroR | Plasmid for cloning of custom gRNA using EnAsCas12a |
| 228938 | pLX313_PELO_WT | Constitutive expression of wild-type human PELO |
| 228940 | pXPR_051d_sgCiChr2-2 | Expression of a CRISPRi control sgRNA that cuts an intergenic region on chromosome 2 |
| 229017 | pXPR_BRD051d_sgCiPELO #1 | Expression of CRISPRi PELO sgRNA #1 |
| 229018 | pXPR_BRD051d_sgCiPELO #2 | Expression of CRISPRi PELO sgRNA #2 |
| 229020 | pRDA355_sgCiPELO #2 | Expression of a CRISPRi doxycycline inducible guide targeting PELO |
| 229021 | pRDA355_sgCiCh2-2 | Expression of a CRISPRi doxycycline inducible control sgRNA that cuts an intergenic region on chromosome 2 |
| 229022 | pRDA355_sgCiPOLR2D | Expression of a CRISPRi doxycycline inducible guide targeting POLR2D |
| 229023 | pRDA052H_sgCh2 | Expression of a EnAsCas12a control guide that cuts an intergenic region on chromosome 2 |
| 229024 | pRDA052H_sgFOCAD #2 | Expression of a EnAsCas12a guide targeting FOCAD |