Mechanism-guided engineering of a minimal biological particle for genome editing.
Ngo W, Peukes J, Baldwin A, Xue ZW, Hwang S, Stickels RR, Lin Z, Satpathy AT, Wells JA, Schekman R, Nogales E, Doudna JA
Proc Natl Acad Sci U S A. 2025 Jan 7;122(1):e2413519121. doi: 10.1073/pnas.2413519121. Epub 2024 Dec 30.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 228957 | pWN_U6-B2M-miniGag | miniEDV production plasmid. Expresses the miniGag (CAG promoter) and sgRNA (human U6 promoter) |
| 228958 | pWN_U6-B2M-miniGag-Cas9 | miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter) |
| 228959 | pWN_U6-TRAC-miniGag-Cas9 | miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter) |
| 228960 | pWN_U6-TRAC-miniGag | miniEDV production plasmid. Expresses the miniGag polypeptide (CAG promoter) and sgRNA (human U6 promoter) |