NEDD4L intramolecular interactions regulate its auto and substrate Na(V)1.5 ubiquitination.
Wright KM, Nathan S, Jiang H, Xia W, Kim H, Chakouri N, Nwafor JN, Fossier L, Srinivasan L, Chen Z, Boronina T, Post J, Paul S, Cole RN, Ben-Johny M, Cole PA, Gabelli SB
J Biol Chem. 2024 Mar;300(3):105715. doi: 10.1016/j.jbc.2024.105715. Epub 2024 Feb 2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 187705 | pET28a(+)-NEDD4L_FL | Express his-tagged NEDD4L FL in bacterial cells |
| 187708 | pET28a(+)-NEDD4L_W2-W3-W4-HECT (381) | Express his-tagged NEDD4L W2-W3-W4-HECT in bacterial cells |
| 187709 | pET28a(+)-NEDD4L_W3-W4-HECT (492) | Express his-tagged NEDD4L W3-W4-HECT in bacterial cells |
| 187710 | pET28a(+)-NEDD4L_W4-HECT | Express his-tagged NEDD4L W4-HECT in bacterial cells |
| 187711 | pET28a(+)-NEDD4L_W3-W4-HECT_C874only | Express his-tagged NEDD4L W3-W4-HECT_C874only in bacterial cells |
| 187712 | pGEX6P1-NEDD4L_FL | Express GST-tagged NEDD4L FL in bacterial cells |
| 187713 | pGEX6P1-NEDD4L_FL-CS | Express the catalytically inactive GST-tagged NEDD4L FL in bacterial cells |
| 187714 | pGEX6P1-NEDD4L_Δ1,2-linker | Express GST-tagged NEDD4L FL with removal of the linker between the WW1 and WW2 domains in bacterial cells |
| 187715 | pGEX6P1-NEDD4L_Δ2,3-linker | Express GST-tagged NEDD4L FL with removal of the linker between the WW2 and WW3 domains in bacterial cells |
| 187716 | pGEX6P1-NEDD4L_W2-W3-W4-HECT (348) | Express GST-tagged NEDD4L W2-W3-W4-HECT (348) in bacterial cells |
| 187718 | pGEX6P1-NEDD4L_WW34 | Express GST-tagged NEDD4L WW34 tandem domains in bacterial cells |
| 187719 | pGEX6P1-NEDD4L_HECT | Express GST-tagged NEDD4L HECT domain in bacterial cells |
| 187720 | pFastBac1-NEDD4L_ΔC2 (157) | Express GST-tagged NEDD4L ΔC2 (157) in insect cells |
| 187721 | pFastBac1-NEDD4L_W2-W3-W4-HECT (349) | Express GST-tagged NEDD4L W2-W3-W4-HECT (349) in insect cells |
| 187722 | pFastBacHTA-NEDD4L_FL | Express His-tagged NEDD4L FL in insect cells |
| 187723 | pFastBacHTA-NEDD4L_C2-W1-L-W2-HECT | Express His-tagged NEDD4L C2-W1-L-W2-HECT chimera in insect cells |
| 187724 | pFastBacHTA-NEDD4L_W1-L-W2-HECT | Express His-tagged NEDD4L W1-L-W2-HECT chimera in inserct cells |
| 187725 | His-Precission-NEDD4L (381-975)_pcDNA3.1(+) | Express his-tagged NEDD4L W2-W3-W4-HECT (381) in mammalian cells |
| 187726 | His-Precission-NEDD4L (492-975)_pcDNA3.1(+) | Express his-tagged NEDD4L W3-W4-HECT in mammalian cells |
| 187727 | His-Precission-NEDD4L_Δ1,2-linker_pcDNA3.1(+) | Express his-tagged NEDD4L FL with removal of the linker between the WW1 and WW2 domains in mammalian cells |
| 187728 | His-Precission-NEDD4L_Δ2,3-linker_pcDNA3.1(+) | Express his-tagged NEDD4L FL with removal of the linker between the WW2 and WW3 domains in mammalian cells |
| 187729 | His-Precission-NEDD4L_C942S_pCDNA3.1(+) | Express the catalytically inactive NEDD4L FL in mammalian cells |
| 187730 | His-Precission-NEDD4L_FL_pCDNA3.1(+) | Express NEDD4L FL in mammalian cells |
| 187732 | Nav1.5_1-2linker411-717_PPSYtoAASA1960-1996 | Express a chimera of the Nav1.5 linker between the first and second transmembrane domain with the motif surrounding PPSY; residues 1974-1977 PPSY mutated to AASA |