Optogenetic tools for inducing organelle membrane rupture.
Nagashima Y, Eguchi T, Koyama-Honda I, Mizushima N
J Biol Chem. 2025 Mar 18;301(4):108421. doi: 10.1016/j.jbc.2025.108421.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 228939 | pMRX-IP-EGFP-PA-BAX(lyso) | Stable expression of EGFP-LOV2(G528A, N538E)-BAX-TMEM106B in mammalian cells. Photoactivatable BAX localizes on lysosomal membrane and induces lysosomal rupture upon blue light stimulation. |
| 228941 | pMRX-IP-EGFP-PA-BAX(ER) | Stable expression of EGFP-LOV2(N538E)-BAX-Cyb5a in mammalian cells. Photoactivatable BAX localizes on ER and induces ER rupture upon blue light stimulation. |
| 228942 | pMRX-IP-EGFP-PA-BAX(mito) | Stable expression of EGFP-LOV2(N538E)-BAX-OMP25 in mammalian cells. Photoactivatable BAX localizes on the outer mitochondrial membrane and induces mitochondrial rupture upon blue light stimulation. |
| 233343 | pMRX-IP-EGFP-PA-BAX(mito, G108V) | Stable expression of EGFP-LOV2(N538E)-BAX(G108V)-OMP25 in mammalian cells. The G108V mutation suppresses pore-forming activity of BAX. |
| 233345 | pMRX-IP-EGFP-PA-BAX(lyso, G108V) | Stable expression of EGFP-LOV2(G528A, N538E)-BAX-TMEM106B in mammalian cells. The G108V mutation suppresses pore-forming activity of BAX. |
| 233346 | pMRX-IP-EGFP-LOV2-OMP25 | Stable expression of EGFP-LOV2(N538E)-OMP25 in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation. |
| 233347 | pMRX-IP-EGFP-LOV2-TMEM106B | Stable expression of EGFP-LOV2(G528A, N538E)-TMEM106B in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation. |
| 233348 | pMRX-IP-EGFP-LOV2-Cyb5a | Stable expression of EGFP-LOV2(N538E)-Cyb5a in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation. |
| 233349 | pMRX-IZ-SEC61B-mCherry-FRB | Stable expression of SEC61B-mCherry-FRB in mammalian cells. FRB localizes in the ER lumen. |
| 233350 | pMRX-IB-FKBP-HaloTag | Stable expression of FKBP-Halotag in mammalian cells. FKBP-halotag localizes in the cytosol and binds to the FRB tag in the presence of AP21967 (A/C Heterodimerizer). |