Mitochondrial pyruvate transport regulates presynaptic metabolism and neurotransmission.
Tiwari A, Myeong J, Hashemiaghdam A, Stunault MI, Zhang H, Niu X, Laramie MA, Sponagel J, Shriver LP, Patti GJ, Klyachko VA, Ashrafi G
Sci Adv. 2024 Nov 15;10(46):eadp7423. doi: 10.1126/sciadv.adp7423. Epub 2024 Nov 15.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 228903 | wt MPC1-FLAG | Expression of wild-type mouse MPC1 protein with a C terminal FLAG tag |
| 228904 | MPC1-AA-FLAG | Expression of mouse MPC1 in which K45 and K46 motif sites were replaced with alanine blocking acetylation with a C terminal FLAG tag |
| 228905 | MPC1-QQ-FLAG | Expression of mouse MPC1 in which K45 and K46 motif sites were replaced with glutamine to mimic acetylated lysines with a C terminal FLAG tag |
| 228906 | CamKII-mtPyronicSF | Expression of mtPyronicSF in neurons targeted to the mitochondria serving as an optical sensor for mitochondrial pyruvate uptake. Backbone from Addgene plasmid 100834 |
| 229016 | pLKO.1-mTagBFP2-rMPC1 shRNA | Expression of an shRNA construct that knocks down rat MPC1. This plasmid also encodes a blue fluorescent protein tag to verify transfection |