High-throughput evaluation of genetic variants with prime editing sensor libraries.
Gould SI, Wuest AN, Dong K, Johnson GA, Hsu A, Narendra VK, Atwa O, Levine SS, Liu DR, Sanchez Rivera FJ
Nat Biotechnol. 2024 Mar 12. doi: 10.1038/s41587-024-02172-9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 228890 | Lenti-EFS-PEmax-P2A-Puro | Lentiviral delivery and expression of PEmax protein with puromycin selection cassette. |
| 228892 | Lenti-PEAR-mCherry | All-in-one lentiviral version of the PEAR reporter. Constitutive RFP expression with GFP turned on in event of prime editing. |
| 228893 | Lenti-Trono-EFS-Blast-P2A-TurboRFP | Cloning and expression of gRNAs or pegRNAs with EcoRI/Esp3I insertion. |
| 228894 | Lenti-UPEmS-tevo | Golden gate cloning (BsmBI) of pegRNAs with tevopreQ1 motif, and with constitutive RFP expression. |