The intracellular bacterium Orientia tsutsugamushi hijacks the adaptor protein BICD2 for dynein-based motility
Manigrasso G, Saharat K, Kullapanich C, Boulanger J, Morgan TE, Kramer H, Salje J, Carter AP
bioRxiv 2024.04.11.589026
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 228823 | pcDNA5/FRT/TO_eGFP-pScaA | For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaA in HeLa Flp-In cells |
| 228824 | pcDNA5/FRT/TO_eGFP-pScaC | For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaC in HeLa Flp-In cells |
| 228825 | pcDNA5/FRT/TO_eGFP-pScaD | For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaD in HeLa Flp-In cells |
| 228826 | pcDNA5/FRT/TO_eGFP-pScaE | For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaE in HeLa Flp-In cells |
| 228827 | pcDNA5/FRT/TO_eGFP-pScaC-MTS | For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaC C-terminally fused to a mitochondrial targeting sequence in HeLa Flp-In cells |
| 228829 | pACEBac1_Strep-Psc-BICD2 | Insect cell expression of BICD2 |
| 228830 | pACEBac1_Strep-Psc-BICD2(1-400) | Insect cell expression of N-terminal portion of BICD2 (aa 1-400) |
| 228831 | pACEBac1_Strep-Psc-BICD2(1-710) | Insect cell expression of truncated BICD2 (aa 1-710) |
| 228832 | pACEBac1_Strep-Psc-BICD2(342-800) | Insect cell expression of C-terminal portion of BICD2 (aa 342-800) |
| 228833 | pACEBac1_Strep-Psc-BICD2(711-800) | Insect cell expression of CC3 domain of BICD2 (aa 711-800) |
| 228835 | pET22b_pScaC-SNAP_6xHis | E. coli expression of the SNAP-tagged ScaC passenger domain |
| 228839 | pACEBac1_Strep-Psc-BICD2(711-800)_L742A | Insect cell expression of the mutant C-terminus of BICD2 |