An APEX2-based proximity-dependent biotinylation assay with temporal specificity to study protein interactions during autophagy in the yeast Saccharomyces cerevisiae.
Filali-Mouncef Y, Leytens A, Vargas Duarte P, Zampieri M, Dengjel J, Reggiori F
Autophagy. 2024 Oct;20(10):2323-2337. doi: 10.1080/15548627.2024.2366749. Epub 2024 Jul 3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 229228 | pFA6a-FLAG-APEX2-NES-TRP1 | Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast |
| 229229 | pFA6a-FLAG-APEX2-NES-K.l.LEU2 | Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast |
| 229231 | pFA6a-FLAG-APEX2-NES-NatMX6 | Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast |