Rational Design of Enhanced Nme2Cas9 and Nme2SmuCas9 Nucleases and Base Editors
Bamidele N, Ansodaria A, Chen Z, Cheng H, Panwala R, Jazbec E, Sontheimer EJ
bioRxiv November 07, 2024
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 228717 | pEJS2696-CMV-Nme2SmuCas9-D56R | Expresses Nme2Smu Cas9 nuclease with D56R mutation in mammalian cells |
| 228718 | pEJS2697-CMV-Nme2SmuCas9-E932R | Expresses Nme2Smu Cas9 nuclease with E932R mutation in mammalian cells |
| 228719 | pEJS2698-CMV-Nme2Cas9-D56R | Expresses Nme2 Cas9 nuclease with D56R mutation in mammalian cells |
| 228720 | pEJS2699-CMV-Nme2Cas9-E932R | Expresses Nme2 Cas9 nuclease with E932R mutation in mammalian cells |
| 228721 | pEJS2700-CMV-Nme2-ABE8e-nt-E932R | Expresses N-terminal ABE8e-Nme2 nCas9 (D16A) base editor with E932R mutation in mammalian cells |
| 228722 | pEJS2701-CMV-Nme2Smu-ABE8e-nt-E932R | Expresses N-terminal ABE8e-Nme2Smu nCas9 (D16A) base editor with E932R mutation in mammalian cells |
| 228723 | pEJS2702-CMV-iNme2-ABE8e-nt | Expresses N-terminal ABE8e-iNme2 nCas9 (D16A) base editor in mammalian cells |
| 228724 | pEJS2703-CMV-iNme2Smu-ABE8e-nt | Expresses N-terminal ABE8e-iNme2Smu nCas9 (D16A) base editor in mammalian cells |
| 228725 | pEJS2704-CMV-Nme2Smu-ABE8e-i8-L10-E932R | Expresses domain inlaid Nme2Smu nCas9 (D16A)-i8-ABE8e base editor with linker-10 and E932R mutation in mammalian cells |
| 228726 | pEJS2705-CMV-Nme2Smu-ABE8e-i8-L10-D56R | Expresses domain inlaid Nme2Smu nCas9 (D16A)-i8-ABE8e base editor with linker-10 and D56R mutation in mammalian cells |
| 228727 | pEJS2706-CMV-Nme2-ABE8e-i8-L10-E932R | Expresses domain inlaid Nme2 nCas9 (D16A)-i8-ABE8e base editor with linker-10 and E932R mutation in mammalian cells |
| 228728 | pEJS2707-CMV-Nme2-ABE8e-i8-L10-D56R | Expresses domain inlaid Nme2 nCas9 (D16A)-i8-ABE8e base editor with linker-10 and D56R mutation in mammalian cells |
| 228729 | pEJS2708-CMV-Nme2Smu-ABE8e-i1-L10-E932R | Expresses domain inlaid Nme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 and E932R mutation in mammalian cells |
| 228730 | pEJS2709-CMV-Nme2Smu-ABE8e-i1-L10-D56R | Expresses domain inlaid Nme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 and D56R mutation in mammalian cells |
| 228731 | pEJS2710-CMV-Nme2-ABE8e-i1-L10-E932R | Expresses domain inlaid Nme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 and E932R mutation in mammalian cells |
| 228732 | pEJS2711-CMV-Nme2-ABE8e-i1-L10-D56R | Expresses domain inlaid Nme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 and D56R mutation in mammalian cells |
| 228733 | pEJS2712-CMV-eNme2-C-ABE8e-i1-L10 | Expresses domain inlaid eNme2-C nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells |
| 228734 | pEJS2713-CMV-iNme2-ABE8e-i1-L10 | Expresses domain inlaid iNme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells |
| 228735 | pEJS2714-CMV-iNme2Smu-ABE8e-i1-L10 | Expresses domain inlaid iNme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells |