Rapid in silico directed evolution by a protein language model with EVOLVEpro
Jiang K, Yan Z, Di Bernardo M, Sgrizzi SR, Villiger L, Kayabolen A, Kim B, Carscadden JK, Hiraizumi M, Nishimasu H, Gootenberg JS, Abudayyeh OO
Science 0, eadr6006
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 228708 | pKJ423 | Mammalian expression of Anti-CD71 LC (light chain); to be used with pKJ455 (Plasmid 228707) to make the antibody |
| 228709 | pKJ458 | Mammalian expression of Antibody C143 HC R39K (heavy chain); to be used with pKJ460 (Plasmid 228710) to make the antibody |
| 228710 | pKJ460 | Mammalian expression of Antibody C143 LC N28R (light chain); to be used with pKJ458 (Plasmid 228709) to make the antibody |
| 228711 | pKJ0376 | Mammalian expression of human codon-optimized Bxb1 mutant N194G |
| 228712 | pKJ0377 | Mammalian expression of human codon-optimized enhanced Bxb1 (epBxb1 mutant T166R) |
| 228713 | pDF850 | Mammalian expression of EVOLVEpro PsaCas12f (epPsaCas12f) |
| 228714 | pKJ247 | Mammalian expression of U6 epPsaCas12f RNF2 targeting guide |
| 228715 | pKJ0463 | Bacterial expression of epT7 RNAP; for protein production and purification |
| 228716 | pKJ0464 | Bacterial expression of T7 RNAP with E643G mutation; for protein production and purification |