Frank von Delft Lab: ASAP Discovery Plasmids 2024
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 228632 | Human Coxsackievirus A16 strain G10 2A protease | Bacterial expression of the Coxsackievirus A16 (G-10) 2A protease this construct can be used for biochemical assay and to obtain crystals in the space group C1 2 1(PDB 8POA) |
| 228634 | Enterovirus A71 (EV-A71) isolate 4643-TW98 2A protease with non-cleavable C-terminal His AviTag | Bacterial expression of the A71 2A protease for assays that require immobilization using biotin-streptavidin interaction |
| 228635 | Enterovirus A71 (EV-A71) isolate 4643-TW98 3C protease with non-cleavable C-terminal His AviTag | Bacterial expression of the A71 3C protease for assays that require immobilization using biotin-streptavidin interaction |
| 228636 | Enterovirus A71 (EV-A71) isolate TW-2553-1998 3C protease | Bacterial expression of the A71 3C protease (isoform B) used in crystallisation |
| 228637 | Coxsackievirus A16 (CVA16) isolate ENT-2012-0948 3C protease | Bacterial expression of the Coxsackievirus 16 3C protease |
| 228638 | Coxsackievirus A6 (CVA6) strain JLS10/HeN/CHN/2019 3C protease | Bacterial expression of the Coxsackievirus 63 3C protease |
| 228639 | Coxsackievirus B3 (CVB3) 3C protease isolate KR-2002 | Bacterial expression of the Coxsackievirus 33 isoform B 3C protease |
| 228640 | Coxsackievirus B4 (CVB4) 3C protease isolate CVB4/USA/9R1/2012 | Bacterial expression of the Coxsackievirus 43 isoform B 3C protease |
| 228641 | SARS-CoV-2 isolate:Wuhan-Hu-1 Nucleocapsid Protein (C-term oligomerization domain) | Bacterial expression of the SARS-CoV-2 N protein C terminal oligomerization domain used for crystallization assays. |
| 228643 | SARS-CoV-2 isolate:Wuhan-Hu-1 nsp3 macrodomain | Bacterial expression of the SARS-CoV-2 nsp3 macrodomain for crystallization of SARS-CoV-2 nsp3 macrodomain in P 4 3 |
| 228644 | Enterovirus D68 (EV-D68) strain EV-D68 STL 2014 12 3C protease | Bacterial expression of the D68 3C protease |
| 228645 | Echovirus 11 (E11 3C) protease A isolate E11-PLA-NECK-23-E0-62-P1-FRA23 | Bacterial expression of the E11 3C protease |
| 228647 | Poliovirus 1 PV1 isolate 09_05_19 3C protease | Bacterial expression of the Polio 3C protease |
| 228648 | DENV-1 strain US/Hawaii/1944 NS2B-NS3 protease fusion with non cleavable linker | Bacterial expression of the fusion of the NS2B-NS3 protease from Dengue virus serotype 1 for biochemical assay |
| 228650 | DENV-2 strain Thailand/16681/84 NS2B-NS3 protease fusion with non cleavable linker | Bacterial expression of the fusion of the NS2B-NS3 protease from Dengue virus serotype 2 for biochemical assays |
| 228651 | DENV-3 strain Phillippines/H87/1956 NS2B-NS3 protease fusion with non cleavable linker | Bacterial expression of the fusion of the NS2B-NS3 protease from Dengue virus serotype 3 for biochemical assays |
| 228652 | DENV-4 strain H241 NS2B-NS3 protease fusion with non cleavable linker | Bacterial expression of the fusion of the NS2B-NS3 protease from Dengue virus serotype 4 for biochemical assays |
| 228653 | MVEV strain MVE-1-51 NS2B-NS3 protease fusion with non cleavable linker | Bacterial expression of the fusion of the NS2B-NS3 protease from Murray Valley encephalitis virus for biochemical assay |
| 228654 | POWV strain LB NS2B-NS3 protease fusion with non cleavable linker | Bacterial expression of the fusion of the NS2B-NS3 protease from Powassan virus for biochemical assay |
| 228655 | ROCV strain SPH 34675 NS2B-NS3 protease fusion with non cleavable linker | Bacterial expression of the fusion of the NS2B-NS3 protease from Rocio virus for biochemical assay |
| 228656 | SPOV strain SM-6 V-1 NS2B-NS3 protease fusion with non cleavable linker | Bacterial expression of the fusion of the NS2B-NS3 protease from Spondweni virus for biochemical assay |
| 228657 | SLEV strain Kern217 NS2B-NS3 protease fusion with non cleavable linker | Bacterial expression of the fusion of the NS2B-NS3 protease from St. Louis encephalitis virus for biochemical assay |
| 228658 | USUV strain Vienna 2001 NS2B-NS3 protease fusion with non cleavable linker | Bacterial expression of the fusion of the NS2B-NS3 protease from Usutu virus for biochemical assay |
| 228659 | WESSV strain SAH177 NS2B-NS3 protease fusion with non cleavable linker | Bacterial expression of the fusion of the NS2B-NS3 protease from Wesselsbron virus for biochemical assay |
| 228660 | WNV strain-NY99 NS2B-NS3 protease fusion with non cleavable linker with mutation K to A to reduce autoproteolysis | Bacterial expression of the fusion of the NS2B-NS3 protease from West Nile virus with an H163A - catalytically inactive mutation for xxxxx |
| 228661 | ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease fusion with non cleavable linker (N-terminal Twin-Strep-tag) | Bacterial expression of the fusion of the NS2B-NS3 protease from Zika virus for biochemical assay |
| 228662 | WNV isolate WN NY 2000-crow3356 NS5 RdRp full length | Bacterial expression of the West Nile virus NS5 RdRp protein for biochemical assay |
| 228663 | ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease co-expression of NS2B and NS3 protease domain | Bacterial co-expression of the NS2B and NS3 proteases from Zika virus for crystallization assays in P43 2 2 (PDB:8PN6) |
| 228664 | ZIKV isolate PRVABC59 NS3 helicase domain | Bacterial expression of the Zika virus NS3 helicase |
| 228665 | Human Coxsackievirus A16 strain G10 inactive 2A protease | Bacterial expression of the A71 2A protease, innactive and can be used as a control in assays |