The LC3B FRET biosensor monitors the modes of action of ATG4B during autophagy in living cells.
Gokerkucuk EB, Cheron A, Tramier M, Bertolin G
Autophagy. 2023 Aug;19(8):2275-2295. doi: 10.1080/15548627.2023.2179845. Epub 2023 Feb 22.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 228559 | Aquamarine-LC3B | Encodes a donor-only, Aquamarine-tagged version of the LC3B biosensor under the control of the CMV promoter |
| 228560 | Aquamarine-LC3B-TdLanYFP | Encodes the LC3B biosensor where LC3B is flanked by the Aquamarine/TdLanYFP donor/accpetor FRET pair and under the control of the CMV promoter |
| 228561 | Aquamarine-LC3B G120A-TdLanYFP | Encodes an uncleavable version of the LC3B biosensor. Mutated LC3B is flanked by the Aquamarine/TdLanYFP donor/accpetor FRET pair and under the control of the CMV promoter |
| 228563 | Aquamarine-LC3B G120A | Encodes a donor-only, Aquamarine-tagged version of the inactive LC3B biosensor under the control of the CMV promoter |
| 228565 | LC3B-TdLanYFP | Encodes an acceptor-only, TdLanYFP-tagged version of the LC3B biosensor under the control of the CMV promoter |