Jeferson Gross Lab: Engineering Adaptive Alleles for Escherichia coli Growth on Sucrose Using the EasyGuide CRISPR System
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 228548 | pSpec3' | Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec5', to clone gRNA plasmid |
| 228549 | pSpec5' | Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec3', to clone gRNA plasmid |
| 228550 | pOliSpec3' | Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec5' and oligo-spacer amplifications, to clone gRNA plasmid. |
| 228551 | pOliSpec5' | Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec3' and oligo-spacer amplifications, to clone gRNA plasmid |
| 228552 | pTargetAmp | Template for PCR amplification for gRNA cloning plasmid by in vivo cloning. |