Michael Boettcher Lab: On the utility of Cas13d for genetic interaction mapping
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 228359 | pXR001-mCD4: EF1a-RfxCas13d-P2A-mCD4-T2A-EGFP | RfxCas13d (NLS-RfxCas13d-NLS) with 2A-EGFP and 2A-mCD4 for RNA knockdown |
| 228360 | 783-Rx-hU6: RfxCas13d gRNA and array cloning backbone | hU6-driven expression of RfxCas13d gRNAs and arrays compatible. Contains AarI sites for guide cloning flanked by 5' DR36 |
| 228361 | 783-Rx-mU6: RfxCas13d gRNA and array cloning backbone | mU6-driven expression of RfxCas13d gRNAs and arrays. Contains AarI sites for guide cloning. |
| 228362 | 783-Rx-Dual: Combinatorial RfxCas13d gRNA and array cloning backbone | mU6- and hU6-driven expression of dual RfxCas13d gRNAs and arrays for combinatorial RNA knockdown. |
| 228363 | pMB1: All in one SpCas9 expression and sgRNA cloning backbone | mU6-driven expression of spCas9 sgRNAs with EF1a-SpCas9-2A-Puro-2A-mCherry |
| 228364 | AiO-Cas12a: All in one enAsCas12a expression and gRNA cloning backbone | mU6-driven expression of enAsCas12a gRNAs with EF1a-enAsCas12a-2A-Puro |