A new generation of nanobody research tools using improved mass spectrometry-based discovery methods.
Fridy PC, Farrell RJ, Molloy KR, Keegan S, Wang J, Jacobs EY, Li Y, Trivedi J, Sehgal V, Fenyo D, Wu Z, Chait BT, Rout MP
J Biol Chem. 2024 Aug 2;300(9):107623. doi: 10.1016/j.jbc.2024.107623.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 228961 | pET21-pelB-LaG94-1 | For bacterial expression of anti-GFP nanobody LaG94-1, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag. |
| 228962 | pET21-pelB-LaG94-2 | For bacterial expression of anti-GFP nanobody LaG94-2, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag. |
| 228963 | pET21-pelB-LaG94-3 | For bacterial expression of anti-GFP nanobody LaG94-3, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag. |
| 228964 | pET21-pelB-LaG94-4 | For bacterial expression of anti-GFP nanobody LaG94-4, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag. |
| 228965 | pET21-pelB-LaG94-5 | For bacterial expression of anti-GFP nanobody LaG94-5, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag. |
| 228966 | pET21-pelB-LaG94-6 | For bacterial expression of anti-GFP nanobody LaG94-6, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag. |
| 228967 | pET21-pelB-LaG94-8 | For bacterial expression of anti-GFP nanobody LaG94-8, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag. |
| 228968 | pET21-pelB-LaG94-9 | For bacterial expression of anti-GFP nanobody LaG94-9, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag. |
| 228969 | pET21-pelB-LaG94-10 | For bacterial expression of anti-GFP nanobody LaG94-10, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag. |
| 228970 | pET21-pelB-LaG94-12 | For bacterial expression of anti-GFP nanobody LaG94-12, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag. |
| 228971 | pET21-pelB-LaG94-15 | For bacterial expression of anti-GFP nanobody LaG94-15, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag. |
| 228972 | pET21-pelB-LaG94-18 | For bacterial expression of anti-GFP nanobody LaG94-18, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag. |
| 228973 | pET21-pelB-LaG94-1--LaG94-14 | For bacterial expression of anti-GFP nanobody dimer of LaG94-1 and LaG94-14, with pelB leader for periplasmic secretion, and C-term 6xHIS tag. |
| 228974 | pET21-pelB-LaTdT-1 | For bacterial expression of anti-tdTomato nanobody LaTdT-1, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag. |
| 228975 | pET21-pelB-LaTdT-2 | For bacterial expression of anti-tdTomato nanobody LaTdT-2, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag. |
| 228976 | pET21-pelB-LaTdT-8 | For bacterial expression of anti-tdTomato nanobody LaTdT-8, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag. |
| 228977 | pET21-pelB-LaTdT-9 | For bacterial expression of anti-tdTomato nanobody LaTdT-9, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag. |
| 228978 | pET21-pelB-LaTdT-10 | For bacterial expression of anti-tdTomato nanobody LaTdT-10, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag. |
| 228979 | pET21-pelB-LaTdT-38 | For bacterial expression of anti-tdTomato nanobody LaTdT-38, with pelB leader for periplasmic secretion, and C-term 6xHIS tag. |
| 228980 | pET21-pelB-LaTdT-39 | For bacterial expression of anti-tdTomato nanobody LaTdT-39, with pelB leader for periplasmic secretion, and C-term 6xHIS tag. |
| 228981 | pET21-pelB-LaTdT-44 | For bacterial expression of anti-tdTomato nanobody LaTdT-44, with pelB leader for periplasmic secretion, and C-term 6xHIS tag. |
| 228982 | pET21-pelB-LaTdT-45 | For bacterial expression of anti-tdTomato nanobody LaTdT-45, with pelB leader for periplasmic secretion, and C-term 6xHIS tag. |
| 228983 | pET21-pelB-GST-1 | For bacterial expression of anti-GST nanobody GST-1, with pelB leader for periplasmic secretion, and C-term 6xHIS tag. |
| 228984 | pET21-pelB-GST-2 | For bacterial expression of anti-GST nanobody GST-2, with pelB leader for periplasmic secretion, and C-term 6xHIS tag. |
| 228985 | pET21-pelB-GST-3 | For bacterial expression of anti-GST nanobody GST-3, with pelB leader for periplasmic secretion, and C-term 6xHIS tag. |
| 228986 | pET21-pelB-GST-4 | For bacterial expression of anti-GST nanobody GST-4, with pelB leader for periplasmic secretion, and C-term 6xHIS tag. |
| 228987 | pET21-pelB-GST-5 | For bacterial expression of anti-GST nanobody GST-5, with pelB leader for periplasmic secretion, and C-term 6xHIS tag. |
| 228988 | pET21-pelB-GST-6 | For bacterial expression of anti-GST nanobody GST-6, with pelB leader for periplasmic secretion, and C-term 6xHIS tag. |
| 228989 | pET21-pelB-GST-7 | For bacterial expression of anti-GST nanobody GST-7, with pelB leader for periplasmic secretion, and C-term 6xHIS tag. |
| 228990 | pET21-pelB-GST-8 | For bacterial expression of anti-GST nanobody GST-8, with pelB leader for periplasmic secretion, and C-term 6xHIS tag. |
| 228991 | pET21-pelB-LaMIgG-5 | For bacterial expression of anti-mouse IgG nanobody LaMIgG-5, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag. |
| 228992 | pET21-pelB-LaMIgG-8 | For bacterial expression of anti-mouse IgG nanobody LaMIgG-8, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag. |
| 228993 | pET21-pelB-LaMIgG-9 | For bacterial expression of anti-mouse IgG nanobody LaMIgG-9, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag. |
| 228994 | pET21-pelB-LaMIgG-14 | For bacterial expression of anti-mouse IgG nanobody LaMIgG-14, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag. |
| 228995 | pET21-pelB-LaRIgG-1 | For bacterial expression of anti-rabbit IgG nanobody LaRIgG-1, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag. |
| 228996 | pET21-pelB-LaRIgG-2 | For bacterial expression of anti-rabbit IgG nanobody LaRIgG-2, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag. |
| 228997 | pET21-pelB-LaRIgG-7 | For bacterial expression of anti-rabbit IgG nanobody LaRIgG-7, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag. |
| 228998 | pET21-pelB-LaGIgG-4 | For bacterial expression of anti-mouse IgG nanobody LaMIgG-4, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag. |
| 228999 | pET21-pelB-LaGIgG-12 | For bacterial expression of anti-mouse IgG nanobody LaMIgG-12, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag. |