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Template plasmids optimized for deletion of multiple genes in yeast Saccharomyces cerevisiae.

Feng Y-H, Song J-Z, Zhu J, Xie Z
Microbiol Spectr. 2024 Oct 10:e0132024. doi: 10.1128/spectrum.01320-24. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
228256SJZ2-TRP1Template plasmid for yeast gene knockout, uses auxotrophic marker TRP1 (Kluyveromyces lactis)
228257SJZ4-NATTemplate plasmid for yeast gene knockout, confers antibiotic resistance to nourseothricin
228261SJZ9-amdSTemplate plasmid for yeast gene knockout, allows the use of acetamide as sole nitrogen source
228262SJZ10-LEU2Template plasmid for yeast gene knockout, uses auxotrophic marker LEU2 (Kluyveromyces lactis)
228263SJZ1Plasmid backbone
228265SJZ4Plasmid backbone
228267SJZ6Plasmid backbone
228268SJZ7Plasmid backbone

Antibodies from Article