Cytosolic-enhanced dark Epac-based FRET sensors allow for intracellular cAMP detection in live cells via FLIM.
Zanetti G, Klarenbeek JB, Jalink K
FEBS Lett. 2024 Dec 31. doi: 10.1002/1873-3468.15093.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 228242 | Epac-S-H326 | mT2Del_EPACdDEPCD_Q270E_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells. |
| 228243 | Epac-S-H327 | mT2Del_EPACdDEPCD_Q270E_M312L_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells. |
| 228244 | Epac-S-H328 | mT2Del_EPACdDEPCD_Q270E_M312L_E325T_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells. |
| 228245 | Epac-S-H329 | mT2Del_EPACdDEPCD_Q270E_E325T_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells. |
| 228246 | Epac-S-H322 | mT2Del_EPACdDEPCD_Q270E_L777A_K778A_tdcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells. |
| 228247 | Epac-S-H323 | mT2Del_EPACdDEPCD_Q270E_M312L_L777A_K778A_tdcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells. |
| 228248 | Epac-S-H324 | mT2Del_EPACdDEPCD_Q270E_M312L_E325T_L777A_K778A_tdcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells. |
| 228249 | Epac-S-H325 | mT2Del_EPACdDEPCD_Q270E_E325T_L777A_K778A_tdcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells. |
| 228250 | Epac-S-H332 | mT2Del_EPACdDEPCD_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells. |