Decoding RNA Metabolism by RNA-linked CRISPR Screening in Human Cells.
Nugent PJ, Park H, Wladyka CL, Chen KY, Bynum C, Quarterman G, Hsieh AC, Subramaniam AR
bioRxiv [Preprint]. 2024 Jul 26:2024.07.25.605204. doi: 10.1101/2024.07.25.605204.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 228230 | pHPHS309 | GFP dropout backbone for sgRNA library cloning |
| 228236 | pHPHS853 | Bxb1 attB_GA mCherry-T2A-puro, Dox-inducible EYFP reporter for integration into Bxb1 attP_GA landing pad |
| 228237 | pHPHS926 | Bxb1 attB_GA mCherry-T2A-puro, Dox-inducible β-globin NMD reporter with premature termination codon for integration into Bxb1 attP_GA landing pad |
| 228238 | pHPHS927 | Bxb1 attB_GA mCherry-T2A-puro, Dox-inducible β-globin reporter for integration into Bxb1 attP_GA landing pad |
| 228240 | pHPHS800 | Bxb1 attB_wildtype site and SpCas9-hygro for integration into Bxb1 attP_wildtype landing pad (eg. pHPHS232). Includes pEF1 and attP_GA for second round of recombination |
| 228356 | pHPHS232 | Landing pad with Bxb1 attP_wildtype site, Dox-inducible BFP-iCasp9-Blasticidin, CMV-driven rtTA3, T2A-neomycin for HDR into AAVS1 locus |