An experimental census of retrons for DNA production and genome editing.
Khan AG, Rojas-Montero M, Gonzalez-Delgado A, Lopez SC, Fang RF, Crawford KD, Shipman SL
Nat Biotechnol. 2024 Sep 17. doi: 10.1038/s41587-024-02384-z.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 228113 | pAGD289 | pET-21(+) deriviative with retron Kva1 containing a donor to edit E. coli rpoB gene cloned downstream of a T7 promoter |
| 228114 | pAGK55 | Express node 2 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells |
| 228116 | pAGK56 | Express node 412 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells |
| 228117 | pAGK57 | Express node 482 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells |
| 228119 | pAGK59 | Express node 1429 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells |
| 228120 | pAGK60 | Express node 1438 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells |
| 228121 | pAGK61 | Express node 1467 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells |
| 228122 | pAGK62 | Express node 1531 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells |
| 228123 | pAGK65 | Express node 1795 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells |
| 228126 | pAGK111 | Express node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) EMX1 gene in human cells |
| 228127 | pAGK112 | Express node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) HEK4 gene in human cells |