Methotrexate-based PROTACs as DHFR-specific chemical probes.
Rana S, Dranchak P, Dahlin JL, Lamy L, Li W, Oliphant E, Shrimp JH, Rajacharya GH, Tharakan R, Holland DO, Whitten AS, Wilson KM, Singh PK, Durum SK, Tao D, Rai G, Inglese J
Cell Chem Biol. 2024 Feb 15;31(2):221-233.e14. doi: 10.1016/j.chembiol.2023.09.014. Epub 2023 Oct 23.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 179731 | pBMN DHFR-HiBiT / Lyt2 | Expresses human dihydrofolate reductase (DHFR) with C-terminal HiBiT tag in mammalian cells. This retroviral vector has an IRES-Lyt-2 downstream of the cloning sites |
| 193883 | pET21a HsDHFR-bio-10His | Expresses human dihydrofolate reductase (DHFR) with C-terminal AviTag (Bio) and His10 sequence |