Characterization of BioID tagging systems in budding yeast and exploring the interactome of the Ccr4-Not complex.
Pfannenstein J, Tyryshkin M, Gulden ME, Doud EH, Mosley AL, Reese JC
G3 (Bethesda). 2024 Sep 13:jkae221. doi: 10.1093/g3journal/jkae221.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 227974 | pFA6a-BirA-HA3-KanMX6 | To insert HA-tagged biotin ligase, BirA, at the C-terminus of genes; yeast BioID with KanMX6 marker |
| 227975 | pFA6a-BirA-HA3-HIS3MX6 | To insert HA-tagged biotin ligase, BirA, at the C-terminus of genes; yeast BioID, HIS3MX6 marker |
| 227976 | pFA6a-BioID2-HA3-KanMX6 | To insert HA-tagged biotin ligase, BioID2, at the C-terminus of genes; yeast BioID, KanMX6 marker |
| 227977 | pFA6a-BioID2-HA3-HIS3MX6 | To insert HA-tagged biotin ligase, BioID2, at the C-terminus of genes; yeast BioID, HIS3MX6 marker |
| 227978 | pFA6a-BASU-HA3-KanMX6 | To insert biotin ligase, BASU, at the C-terminus of genes; yeast BioID, KanMX6 marker |
| 227979 | pFa6-BASU-HA3-HIS3MX | To insert biotin ligase, BASU, at the C-terminus of genes; yeast BioID, HIS3MX marker |
| 227980 | pFA6a-TID-HA3-KanMX6 | To insert biotin ligase, TurboID, at the C-terminus of genes; yeast BioID, KanMX6 marker |
| 227981 | pFA6a-TID-HA3-HIS3MX6 | To insert biotin ligase, TurboID, at the C-terminus of genes; yeast BioID, HIS3MX6 marker |
| 227982 | pFA6a-mTID-HA3-KanMX6 | To insert biotin ligase, miniTurboID, at the C-terminus of genes; yeast BioID, KanMX6 marker |
| 227983 | pFA6a-mTID-HA3-HIS3MX6 | To insert biotin ligase, miniTurboID, at the C-terminus of genes; yeast BioID,HIS3MX6 marker |
| 227984 | pRS416-ADH1p-BirA-FRB-HA3 | Expresses BirA-FRB fusion from ADH1 promoter; yeast BioID |
| 227985 | pRS416-ADH1p-BirA-FRB-NLS-HA3 | Expresses BirA-FRB-NLS fusion from ADH1 promoter; yeast BioID |
| 227986 | pRS416-ADH1p-BirA-FKBP-HA3 | Expresses BirA-FKBP fusion from ADH1 promoter; yeast BioID |
| 227987 | pRS416-ADH1p-BirA-FKBP-NLS-HA3 | Expresses BirA-FKBP-NLS fusion from ADH1 promoter; yeast BioID |
| 227988 | pRS416-ADH1p-BioID2-FRB-HA3 | Expresses BioID2-FRB fusion from ADH1 promoter; yeast BioID |
| 227989 | pRS416-ADH1p-BioID2-FRB-NLS-HA3 | Expresses BioID2-FRB-NLS fusion from ADH1 promoter; yeast BioID |
| 227990 | pRS416-ADH1p-BioID2-FKBP-HA3 | Expresses BioID2-FKBP fusion from ADH1 promoter; yeast BioID |
| 227991 | pRS416-ADH1p- BioID2-FKBP-NLS-HA3 | Expresses BioID2-FKBP-NLS fusion from ADH1 promoter; yeast BioID |
| 227992 | pRS416-ADH1p-BASU-FRB-HA3 | Expresses BASU-FRB fusion from ADH1 promoter; yeast BioID |
| 227993 | pRS416-ADH1p- BASU-FRB-NLS-HA3 | Expresses BASU-FRB-NLS fusion from ADH1 promoter; yeast BioID |
| 227994 | pRS416-ADH1p- BASU-FKBP-HA3 | Expresses BASU-FKBP fusion from ADH1 promoter; yeast BioID |
| 227995 | pRS416-ADH1p- BASU-FKBP-NLS-HA3 | Expresses BASU-FKBP-NLS fusion from ADH1 promoter; yeast BioID |
| 227996 | pRS416-ADH1p-TID-FRB-HA3 | Expresses TurboID-FRB fusion from ADH1 promoter; yeast BioID |
| 227997 | pRS416-ADH1p-TID-FRB-NLS-HA3 | Expresses TurboID-FRB-NLS fusion from ADH1 promoter; yeast BioID |
| 227998 | pRS416-ADH1p-TID-FKBP-HA3 | Expresses TurboID-FKBP fusion from ADH1 promoter; yeast BioID |
| 227999 | pRS416-ADH1p-TID-FKBP-NLS-HA3 | Expresses TurboID-FKBP-NLS fusion from ADH1 promoter; yeast BioID |
| 228000 | pRS416-ADH1p-mTID-FRB-HA3 | Expresses miniTurboID-FRB fusion from ADH1 promoter; yeast BioID |
| 228001 | pRS416-ADH1p-mTID-FRB-NLS-HA3 | Expresses miniTurboID-FRB-NLS fusion from ADH1 promoter; yeast BioID |
| 228002 | pRS416-ADH1p-mTID-FKBP-HA3 | Expresses miniTurboID-FKBP fusion from ADH1 promoter; yeast BioID |
| 228003 | pRS416-ADH1p-mTID-FKBP-NLS-HA3 | Expresses miniTurboID-FKBP-NLS fusion from ADH1 promoter; yeast BioID |
| 228004 | pRS416-HIS3p-TID-FRB-HA3 | Expresses TurboID-FRB fusion from HIS3 promoter; yeast BioID |
| 228005 | pRS416-HIS3p-TID-FRB-NLS-HA3 | Expresses TurboID-FRB-NLS fusion from HIS3 promoter; yeast BioID |
| 228006 | pRS416-HIS3p-TID-FKBP-HA3 | Expresses TurboID-FKBP fusion from HIS3 promoter; yeast BioID |
| 228007 | pRS416-HIS3p- TID-FKBP-NLS-HA3 | Expresses TurboID-FKBP-NLS fusion from HIS3 promoter; yeast BioID |
| 228008 | pRS416-CUP1p-TID-FRB-HA3 | Expresses TurboID-FRB fusion from CUP1 promoter; yeast BioID |
| 228009 | pRS416-CUP1p-TID-FRB-NLS-HA3 | Expresses TurboID-FRB-NLS fusion from CUP1 promoter; yeast BioID |
| 228010 | pRS416-CUP1p-TID-FKBP-HA3 | Expresses TurboID-FKBP fusion from CUP1 promoter; yeast BioID |
| 228011 | pRS416-CUP1p- TID-FKBP-NLS-HA3 | Expresses TurboID-FKBP-NLS fusion from CUP1 promoter; yeast BioID |
| 228012 | pRS416-CHA1p-TID-FRB-HA3 | Expresses TurboID-FRB fusion from CHA1 promoter; yeast BioID |
| 228013 | pRS416-CHA1p-TID-FRB-NLS-HA3 | Expresses TurboID-FRB-NLS fusion from CHA1 promoter; yeast BioID |
| 228014 | pRS416-CHA1p-TID-FKBP-HA3 | Expresses TurboID-FKBP fusion from CHA1 promoter; yeast BioID |
| 228015 | pRS416-CHA1p- TID-FKBP-NLS-HA3 | Expresses TurboID-FKBP-NLS fusion from CHA1 promoter; yeast BioID |
| 228016 | pRS416-MET25p-TID-FRB-HA3 | Expresses TurboID-FRB fusion from MET25 promoter; yeast BioID |
| 228017 | pRS416-MET25p-TID-FRB-NLS-HA3 | Expresses TurboID-FRB-NLS fusion from MET25 promoter; yeast BioID |
| 228018 | pRS416-MET25p-TID-FKBP-HA3 | Expresses TurboID-FKBP fusion from MET25 promoter; yeast BioID |
| 228019 | pRS416-MET25p- TID-FKBP-NLS-HA3 | Expresses TurboID-FKBP-NLS fusion from MET25 promoter; yeast BioID |
| 228020 | pFa6-TID-FRB-3HA-KanMX6 | For TurboID-FRB integration; yeast BioID with KanMX6 marker |
| 228021 | pFa6-TID-FRB-3HA-HIS3MX6 | For TurboID-FRB integration; yeast BioID with HIS3MX6 marker |
| 228022 | pFa6-TID-FRB-NLS-3HA-KanMX6 | To integrate TID-FRB-NLS fusion at yeast promoter; yeast BioID with KanMX6 marker |
| 228023 | pFa6-TID-FRB-NLS-3HA-HIS3MX6 | To integrate TID-FRB-NLS fusion at yeast promoter; yeast BioID with HIS3MX6 marker |
| 228024 | pFa6-TID-FKBP-3HA-KanMX6 | To integrate TID-FKBP fusion at yeast promoter; yeast BioID with KanMX6 marker |
| 228025 | pFa6-TID-FKBP-3HA-HIS3MX6 | To integrate TID-FKBP fusion at yeast promoter; yeast BioID with HIS3MX6 marker |
| 228026 | pFa6-TID-FKBP-NLS-3HA-KanMX6 | To integrate TID-FKBP-NLS fusion at yeast promoter; yeast BioID with KanMX6 marker |
| 228027 | pFa6-TID-FKBP-NLS-3HA-HISMX6 | To integrate TID-FKBP-NLS fusion at yeast promoter; yeast BioID with HISMX6 marker |