Syn-tasiR-VIGS: virus-based targeted RNAi in plants by synthetic trans-acting small interfering RNAs derived from minimal precursors
Cisneros AE, Alarcia A, Llorens-Gámez JJ, Puertes A, Juárez-Molina M, Primc A, Carbonell A
Nucleic Acid Research 2025
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 227962 | pENTR-B/c | Entry plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs. |
| 227963 | pMDC32B-B/c | Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs. |
| 227964 | pENTR-AtmiR173aTS-B/c | Entry plasmid with AtmiR173aTS and a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs. |
| 227965 | pMDC32B-AtmiR173aTS-B/c | Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. For expressing syn-tasiRNAs in Arabidopsis thaliana. |
| 227966 | pENTR-NbmiR482aTS-B/c | Entry plasmid with NbmiR482aTS and a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs. |
| 227967 | pMDC32B-NbmiR482aTS-B/c | Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. For expressing syn-tasiRNAs in Nicotiana benthamiana. |