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Global organelle profiling reveals subcellular localization and remodeling at proteome scale.

Hein MY, Peng D, Todorova V, McCarthy F, Kim K, Liu C, Savy L, Januel C, Baltazar-Nunez R, Sekhar M, Vaid S, Bax S, Vangipuram M, Burgess J, Njoya L, Wang E, Ivanov IE, Byrum JR, Pradeep S, Gonzalez CG, Aniseia Y, Creery JS, McMorrow AH, Sunshine S, Yeung-Levy S, DeFelice BC, Mehta SB, Itzhak DN, Elias JE, Leonetti MD
Cell. 2024 Dec 26:S0092-8674(24)01344-8. doi: 10.1016/j.cell.2024.11.028. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
227890pML531_TOMM20-C-term_XTEN80-mEGFP-3xHA_pTMPLTHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
227891pML346_VPS35-C-term_XTEN80-mEGFP-3xHA_pTMPLTHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
227892pML530_EEA1-N-term_XTEN80-mEGFP-3xHA_pTMPLTHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
227893pML374_CLTA-N-term_XTEN80-mEGFP-3xHA_pTMPLTHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
227894pML307_SEC61B-N-term_XTEN80-mEGFP-3xHA_pTMPLTHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
227896pML383_CCDC47-C-term_XTEN80-mEGFP-3xHA_pTMPLTHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
227897pML384_NCLN-C-term_XTEN80-mEGFP-3xHA_pTMPLTHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
227898pML333_LAMP1-C-term_XTEN80-mEGFP-3xHA_pTMPLTHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
227899pML334_RAB7A-N-term_XTEN80-mEGFP-3xHA_pTMPLTHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
227900pML348_ATG101-N-term_XTEN80-mEGFP-3xHA_pTMPLTHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
227901pML460_MAP1LC3B-N-term_XTEN80-mEGFP-3xHA_pTMPLTHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
227902pML349_PEX3-C-term_XTEN80-mEGFP-3xHA_pTMPLTHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
227903pML318_SEC23A-N-term_XTEN80-mEGFP-3xHA_pTMPLTHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
227904pML319_SEC31A-N-term_XTEN80-mEGFP-3xHA_pTMPLTHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
227905pML317_COPE-C-term_XTEN80-mEGFP-3xHA_pTMPLTHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
227907pML340_RAB11A-N-term_XTEN80-mEGFP-3xHA_pTMPLTHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
227908pML343_RAB14-N-term_XTEN80-mEGFP-3xHA_pTMPLTHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
227909pML325_GPR107-C-term_XTEN80-mEGFP-3xHA_pTMPLTHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
227910pML323_GOLGA2-N-term_XTEN80-mEGFP-3xHA_pTMPLTHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
227911pML600_COG8-C-term_XTEN80-mEGFP-3xHA_pTMPLTHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
227912pML494_ATP1B3-C-term_XTEN80-sfGFP-3xHA_pTMPLTHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
227913pML499_SLC3A2-C-term_XTEN80-sfGFP-3xHA_pTMPLTHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
227914pML357_CAV1-C-term_XTEN80-mEGFP-3xHA_pTMPLTHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
227915pML454_G3BP1-N-term_XTEN80-mEGFP-3xHA_pTMPLTHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
227916pML359_CAPRIN1-N-term_XTEN80-mEGFP-3xHA_pTMPLTHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
227918pML361_DCP1A-N-term_XTEN80-mEGFP-3xHA_pTMPLTHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
227920pML366_HSPA1B-N-term_XTEN80-mEGFP-3xHA_pTMPLTHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
227921pML365_HSP90AA1-N-term_XTEN80-mEGFP-3xHA_pTMPLTHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
227922pML364_PSMB7-C-term_XTEN80-mEGFP-3xHA_pTMPLTHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
227923pML484_RPL36-C-term_XTEN80-mEGFP-3xHA_pTMPLTHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
227925pML390_YWHAQ-C-term_XTEN80-mEGFP-3xHA_pTMPLTHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
227926pML389_YWHAB-C-term_XTEN80-mEGFP-3xHA_pTMPLTHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Antibodies from Article