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Light-directed evolution of dynamic, multi-state, and computational protein functionalities

Gligorovski V, Labagnara M, Rahi SJ
bioRxiv 2024.02.28.582517 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
227746pVG117Expresses a version of light-inducible transcription factor EL222 (Asp18Tyr, Val139Leu) under a strong constitutive PGK1pr
227747pVG118Expresses a version of light-inducible transcription factor EL222 (Asp18Tyr) under a strong constitutive PGK1pr
227748pVG119Expresses a version of light-inducible transcription factor EL222 (Val139Leu) under a strong constitutive PGK1pr
227749pVG135Expresses a version of light-inducible transcription factor EL222 (Lys99Glu) under a strong constitutive PGK1pr
227750pVG136Expresses a version of light-inducible transcription factor EL222 (Pro165Ser) under a strong constitutive PGK1pr
227751pVG137Expresses a version of light-inducible transcription factor EL222 (Val201Leu) under a strong constitutive PGK1pr
227752pVG120Expresses a version of light-inducible transcription factor EL222 (Asp89Tyr) under a strong constitutive PGK1pr
227753pVG147Expresses a version of light-inducible transcription factor EL222 (Gly94Cys) under a strong constitutive PGK1pr
227754pVG143Expresses a version of light-inducible transcription factor EL222 (Leu87Ile) under a strong constitutive PGK1pr
227755pVG123Expresses a version of light-inducible transcription factor EL222 (Ala157Val) under a strong constitutive PGK1pr
227757pVG121Expresses a version of light-inducible transcription factor EL222 (Gly82Val) under a strong constitutive PGK1pr
227758pVG124Expresses a version of light-inducible transcription factor EL222 (Thr83Ser) under a strong constitutive PGK1pr
227759pVG150Expresses a version of light-inducible transcription factor EL222 (Ser137Asn) under a strong constitutive PGK1pr
227760pVG144Expresses a version of light-inducible transcription factor EL222 (Val121Met) under a strong constitutive PGK1pr
227761pVG178Expresses a version of light-inducible transcription factor EL222 (Glu84Lys) under a strong constitutive PGK1pr
227770pVG134Expresses a version of light-inducible transcription factor EL222 (Glu84Gly) under a strong constitutive PGK1pr
227771pVG329For genomic replacement of YOR1 gene in budding yeast

Antibodies from Article