When less is more - a fast TurboID knock-in approach for high-sensitivity endogenous interactome mapping.
Stockhammer A, Spalt C, Klemt A, Benz LS, Harel S, Natalia V, Wiench L, Freund C, Kuropka B, Bottanelli F
J Cell Sci. 2024 Aug 15;137(16):jcs261952. doi: 10.1242/jcs.261952. Epub 2024 Aug 28.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 227734 | AP1muA-TurboID-polyA-G418 HR | Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells. |
| 230030 | AP1muA single guide RNA | guide RNA for AP1muA tagging at the C-terminus in pX459 |