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Optimized dicot prime editing enables heritable desired edits in tomato and Arabidopsis.

Vu TV, Nguyen NT, Kim J, Song YJ, Nguyen TH, Kim JY
Nat Plants. 2024 Sep 6. doi: 10.1038/s41477-024-01786-w. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
227692pICH47742::p35S-ePEmax3::tEURb7Golden Gate level 1, position 2, ePEmax3 expression cassette.
227693pICH47742::p35S-PE2max-NC::tEURb7Golden Gate level 1, position 2, PE2max-NC expression cassette.
227694pICH47742:: pRPS5a-PE2max-NC::tHSPGolden Gate level 1, position 2, PE2max-NC expression cassette for Arabidopsis.
227695pICH41331::pU6cm-pegR::EURb7Golden Gate level 0 acceptor vector for cloning altered epegRNA: altered epegRNA being driven by U6 composite promoter (pU6cm) and terminated by a dual terminator (EURb7).
227697pLSL.R.LyReengineered geminiviral replicon vector

Antibodies from Article