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Scot Wolfe Lab: Increasing intracellular dNTP levels improves prime editing efficiency

Unpublished

Plasmids from Article

ID Plasmid Purpose
227673HEK4-53bp-tag-insertion-pegRNAPegRNA for 53 bp insertion
227674PEmax_2star_IVTplasmid for PEmax** mRNA IVT
227675PEmax_2star_pet21AExpression plasmid for PEMAX** protein in bacteria.
227678PEmaxNG-_2star_IVTplasmid for SpCas9-NG-PEmax** mRNA IVT
227679VPX-mRNAplasmid for VPX mRNA IVT

Antibodies from Article