Increasing intracellular dNTP levels improves prime editing efficiency.
Liu P, Ponnienselvan K, Nyalile T, Oikemus S, Joynt AT, Iyer S, Kelly K, Guo D, Kyawe PP, Vanderleeden E, Redick SD, Huang L, Chen Z, Lee JM, Schiffer CA, Harlan DM, Wang JP, Emerson CP Jr, Lawson ND, Watts JK, Sontheimer EJ, Luban J, Wolfe SA
Nat Biotechnol. 2024 Sep 25. doi: 10.1038/s41587-024-02405-x.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 227673 | HEK4-53bp-tag-insertion-pegRNA | PegRNA for 53 bp insertion |
| 227674 | PEmax_2star_IVT | plasmid for PEmax** mRNA IVT |
| 227675 | PEmax_2star_pet21A | Expression plasmid for PEMAX** protein in bacteria. |
| 227678 | PEmaxNG-_2star_IVT | plasmid for SpCas9-NG-PEmax** mRNA IVT |
| 227679 | VPX-mRNA | plasmid for VPX mRNA IVT |