CIFR (Clone–Integrate–Flip-out–Repeat): a toolset for iterative genome and pathway engineering of Gram-negative bacteria
Filippo Federici, Francesco Luppino, Clara Aguilar-Vilar, Maria Eleni Mazaraki, Lars Boje Petersen, Linda Ahonen, Pablo I. Nikel
bioRxiv
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 227668 | pFNC-4 | Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. SmR, easily curable via sucrose counterselection. |