Metabolic perturbations to an E. coli-based cell-free system reveal a trade-off between transcription and translation
Kapasiawala M, Murray RM
bioRxiv 2023.03.22.533877
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 227645 | PTet-F30-Pepper aptamer-UTR1-mTurq2 | pTet-driven expression of F30-scaffolded Pepper aptamer and mTurquoise2 for quantifying cell-free RNA and protein synthesis. The mTurq2 has a GGGGS linker followed by a His6x tag. |
| 227646 | pBEST-PT7extended-MGapt-UTR1-deGFP | pT7-driven expression of MGapt and deGFP for quantifying cell-free RNA and protein synthesis with extended T7 promoter. |
| 227647 | pBEST-PTet-MGapt-UTR1-deGFP | pTet-driven expression of MGapt and deGFP for quantifying cell-free RNA and protein synthesis. |
| 227648 | pBEST-PrOR1OR2-MGapt-UTR1-deGFP | PrOR1OR2-driven expression of MGapt and deGFP for quantifying cell-free RNA and protein synthesis. |