Identification of novel genetic factors that regulate c-di-AMP production in Staphylococcus aureus using a riboswitch-based biosensor.
Kviatkovski I, Zhong Q, Vaidya S, Grundling A
mSphere. 2024 Sep 17:e0032124. doi: 10.1128/msphere.00321-24.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 227636 | pCN38-ktrA-yfp | c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus. |
| 227637 | pCN38-yfp | Negative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus. |
| 227638 | pCN34e-kimA-yfp | c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding kimA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus. |
| 227640 | pCN34e-ktrA-yfp | c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus. |
| 227641 | pCN34e-yfp | Negative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus. |