Autologous DNA mobilization and multiplication expedite natural products discovery from bacteria.
Xie F, Zhao H, Liu J, Yang X, Neuber M, Agrawal AA, Kaur A, Herrmann J, Kalinina OV, Wei X, Muller R, Fu C
Science. 2024 Dec 13;386(6727):eabq7333. doi: 10.1126/science.abq7333. Epub 2024 Dec 13.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 227507 | pCap-101-Apr | Capture plasmid with apramycin resistance and pIJ101 replicon |
| 227508 | pCap-101-Hgy | Capture plasmid with hygromycin resistance and pIJ101 replicon |
| 227579 | pCap-SG5-Apr | Capture plasmid with apramycin resistance and pSG5 replicon |
| 227580 | pCap-SG5-Hgy | Capture plasmid with hygromycin resistance and pSG5 replicon |
| 227581 | pRel | Release plasmid with Cas9 working cassette, apramycin resistance, and pSG5 replicon |
| 227582 | pRel-Hyg | Release plasmid with Cas9 working cassette, hygromycin resistance, and pSG5 replicon |
| 227583 | pHelp | Accessory plasmid with a sgRNA cassette for Cas9, and ampicillin resistance |