Genetically encoded biosensor for fluorescence lifetime imaging of PTEN dynamics in the intact brain.
Kagan T, Gabay M, Meenakshisundaram A, Levi Y, Eid S, Malchenko N, Maman M, Nitzan A, Ravotto L, Zaidel-Bar R, Eickholt BJ, Gal M, Laviv T
Nat Methods. 2025 Apr;22(4):764-777. doi: 10.1038/s41592-025-02610-9. Epub 2025 Feb 20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 227433 | CMV_R-PTEN | Expresses the R-PTEN sensor under a CMV promoter |
| 227434 | CMV_G-PTEN_4A | Expresses the G-PTEN sensor with a 4A mutation |
| 227435 | CMV_R-PTEN_4A | Expression of the R-PTEN sensor with a 4A mutation |
| 227436 | CAG_G-PTEN | Expresses the G-PTEN sensor under a CAG promoter |
| 227438 | AAV-FLEX-CAG_G-PTEN | An AAV backbone for expression of the G-PTEN sensor under the CAG promoter, in a Cre dependent manner. |
| 227440 | PX330-PTEN | Cas9 expression and a gRNA targeting Exon 6 of mouse PTEN |