Nuclear poly-glutamine aggregates rupture the nuclear envelope and hinder its repair.
Korsten G, Osinga M, Pelle RA, Serweta AK, Hoogenberg B, Kampinga HH, Kapitein LC
J Cell Biol. 2024 Nov 4;223(11):e202307142. doi: 10.1083/jcb.202307142. Epub 2024 Aug 16.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 231890 | mCherry-NLS-mCherry | mCherry-tagged nuclear rupture marker |
| 231891 | GFP-polyQ23-NLS | Expression of non-aggregated HTT partial exon 1 Q23-NLS in the nucleus |
| 231892 | GFP-polyQ74-NLS | Forms nuclear HTT partial exon 1 Q74-NLS aggregates |
| 231894 | HaloTag-LaminA | Expression of HaloTag-tagged LaminA to visualize the nuclear lamina |
| 231895 | mCherry-cGAS-HA | Expression of cyclic GMP/AMP synthase (cGAS), as a nuclear rupture marker |
| 231896 | emiRFP670-cGAS | Expression of cyclic GMP/AMP synthase (cGAS), as a nuclear rupture marker |