A precise and sustainable doxycycline-inducible cell line development platform for reliable mammalian cell engineering with gain-of-function mutations.
Shin SW, Min H, Kim J, Lee JS
Metab Eng. 2024 Sep 5;86:12-28. doi: 10.1016/j.ymben.2024.09.004.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
227245 | EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [C12orf35] | All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in CHO cell |
227246 | EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1] | All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cell |