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A precise and sustainable doxycycline-inducible cell line development platform for reliable mammalian cell engineering with gain-of-function mutations.

Shin SW, Min H, Kim J, Lee JS
Metab Eng. 2024 Sep 5;86:12-28. doi: 10.1016/j.ymben.2024.09.004. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
227245EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [C12orf35]All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in CHO cell
227246EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cell

Antibodies from Article