A benchmarked, high-efficiency prime editing platform for multiplexed dropout screening.
Cirincione A, Simpson D, Yan W, McNulty R, Ravisankar P, Solley SC, Yan J, Lim F, Farley EK, Singh M, Adamson B
Nat Methods. 2024 Nov 19. doi: 10.1038/s41592-024-02502-4.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 227597 | pAC025 | Lentiviral backbone used when cloning pooled prime editing guide RNA libraries already containing a tevopreQ1 RNA motif |
| 227598 | pAC026 | Lentiviral backbone used when cloning pooled prime editing guide RNA libraries that do not already contain a tevopreQ1 RNA motif (backbone contains motif) |
| 227706 | StopPR epegRNA library (lAC002) | CRISPR guide RNA library for prime editing |
| 227707 | Self-targeting epegRNA library, +5 G>H (lDS004) | CRISPR guide RNA library for prime editing |
| 227708 | Self-targeting epegRNA library, Tiled edits (lRM001) | CRISPR guide RNA library for prime editing |