Programmable de novo designed coiled coil-mediated phase separation in mammalian cells.
Ramsak M, Ramirez DA, Hough LE, Shirts MR, Vidmar S, Elersic Filipic K, Anderluh G, Jerala R
Nat Commun. 2023 Dec 2;14(1):7973. doi: 10.1038/s41467-023-43742-w.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 228067 | mCit(S1-S3)3-SAH | Mammalian expression of coiled coil segments (S1-S3)x3, with long rigid linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells. |
| 228068 | TagBFP(S2)3(S4)3-gs | Mammalian expression of coiled coil segments (S2)x3-(S4)x3, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells. |
| 228069 | TagBFP(S2)3(S4)3-SAH | Mammalian expression of coiled coil segments (S2)x3-(S4)x3, with long rigid linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells. |
| 228070 | mCit(S1-S3)3-gs | Mammalian expression of coiled coil segments (S1-S3)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells. |
| 228072 | TagBFP(S2)2(S4)2-gs | Mammalian expression of coiled coil segments (S2)x2-(S4)x2, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells. |
| 228073 | mCit(S1-S3)1-SAH | Mammalian expression of coiled coil segments (S1-S3)x1, with long rigid linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells. |
| 228074 | TagBFP(S2-S4)1-gs | Mammalian expression of coiled coil segments (S2-S4)x1, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells. |
| 228075 | TagBFP(S2-S4)3-gs | Mammalian expression of coiled coil segments (S2-S4)x3, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells. |
| 228076 | mCit(S1h-S3h)3-gs | Mammalian expression of coiled coil segments (S1h-S3h)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells. |
| 228077 | TagBFP(S2h)3(S4h)3-gs | Mammalian expression of coiled coil segments (S2h)x3-(S4h)x3, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells. |
| 228079 | mCit(S1h)3(S3h)3-gs | Mammalian expression of coiled coil segments (S1h)x3-(S3h)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells. |
| 228080 | mCit(P5f-P13f)3-gs | Mammalian expression of coiled coil segments (P5f-P13f)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells. |
| 228081 | TagBFP(P6f)3(P14f)3-gs | Mammalian expression of coiled coil segments (P6f)x3-(P14f)x3, with short flexible linkers and N-term TagBFP; for phase separation and forming of liquid condensates in mammalian cells. |
| 228083 | TagBFP(S2h)3(S4h)3-gs-P6f | Mammalian expression of coiled coil segments (S2h)x3-(S4h)x3, with short flexible linkers, N-term TagBFP, and C-term P6f; for phase separation and forming of liquid condensates in mammalian cells. |
| 228084 | mCit(S1h)8-gs | Mammalian expression of coiled coil segments (S1h)x8, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells. |
| 228086 | TagBFP(S2h)8-gs | Mammalian expression of coiled coil segments (S2h)x8, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells. |
| 228087 | TagBFP(S2h)12-gs | Mammalian expression of coiled coil segments (S2h)x12, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells. |
| 228088 | mCit(D1h-D3h)3-gs | Mammalian expression of coiled coil segments (D1h-D3h)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells. |
| 228090 | mCit-APPAP-gs | Mammalian expression of five coiled coil segments in APPAP arrangment, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells. |
| 228091 | mCit-APPAPAPPAP-gs | Mammalian expression of 10 coiled coil segments in APPAPAPPAP arrangement, with short flexible linkers and N-terminal mCitrine for phase separation and forming of liquid condensates in mammalian cells |
| 228092 | RFP-PPPPPPAAAA-gs | Mammalian expression of 10 coiled coil segments in PPPPPPAAAA arrangement, with short flexible linkers and N-terminal mCitrine for phase separation and forming of liquid condensates in mammalian cells |