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Chris Hill Lab: Hill lab spt6-tsh2 plasmids

Unpublished

Plasmids from Article

ID Plasmid Purpose
227084CPH3375_SF_Spt6p-tSH2-1223-1451_NLSGal1p dependent budding yeast expression of tandem-affinity tagged spt6p tSH2 domain (1123-1451) localized to nucleus
227085CPH3566_GST-TEV-Spt6p-tSH2-1223-1451Bacterial expression of GST-tagged Spt6 tSH2 domain (1223-1451)
227086CPH3747_GST-TEV-Spt6p-tSH2-1223-1452Bacterial expression of R1282H single-mutant GST-tagged Spt6 tSH2 domain (1223-1452)
227088CPH3736_GST-TEV-Spt6p-tSH2-1223-1454Bacterial expression of R1282H/K1355A/K1435A triple-mutant GST-tagged Spt6 tSH2 domain (1223-1454)
227089CPH3949_HIS-MBP-TEV-Tom1p-HECT-2850-3268Bacterial expression of HIS-MBP-TEV tagged Tom1p HECT domain (2850-3268)

Antibodies from Article