Engineering IscB to develop highly efficient miniature editing tools in mammalian cells and embryos.

Xue N, Hong D, Zhang D, Wang Q, Zhang S, Yang L, Chen X, Li Y, Han H, Hu C, Liu M, Song G, Guan Y, Wang L, Zhu Y, Li D
Mol Cell. 2024 Aug 22;84(16):3128-3140.e4. doi: 10.1016/j.molcel.2024.07.007. Epub 2024 Aug 2. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
227015	IscB-v3	Vector encoding human codon-optimized enhanced-activity OgeuIscB driven by CMV promoter
227016	eIscB	Vector encoding human codon-optimized enhanced-activity OgeuIscB fusion with extra SV40 NLS driven by CMV promoter
227017	eIscB-D	Vector encoding human codon-optimized enhanced-activity OgeuIscB fusion with HMD-D domain and extra SV40 NLS driven by CMV promoter
227018	TadA-8e-eIscBn	Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CMV promoter
227143	TadA-8e-eIscBn-N-HMG-D	Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e and HMG-D domain in N terminus driven by CMV promoter
227144	eiABE	Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e and HMG-D domain was inserted between TadA-8e and eIscBn driven by CMV promoter
227145	TadA-8e-eIscBn-C-HMG-D	Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e and HMG-D domain in C terminus driven by CMV promoter
227146	hA3A*-IscBn	Vector encoding human codon-optimized nOgeuIscB fusion with two copies of UGI and hA3A* variant driven by CMV promoter
227147	hA3A*-eIscBn-D	Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with two copies of UGI and hA3A* variant and HMG-D domain was inserted between hA3A* and eIscBn driven by CMV promoter
227148	eiCBE	Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with two copies of UGI and hA3A* variant driven by CMV promoter