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Charles Gersbach Lab: Gersbach Lab Cas9 Plasmids 2024

Unpublished

Plasmids from Article

ID Plasmid Purpose
227005pcDNA-Sga-Cas9Mammalian expression of Nuclease active S. gallolyticus Cas9
227006pcDNA-Sin-Cas9Mammalian expression of Nuclease active S. iniae Cas9
227007pcDNA-Spa-Cas9Mammalian expression of Nuclease active S. parasanguinis Cas9
227008pcDNA-Sub-Cas9Mammalian expression of Nuclease active S. uberis Cas9
227009pFUGW hUbC-Sga-dCas9-KRAB-T2A-GFPLentiviral Expression of dCas9-KRAB from S. gallolyticus fused to the repressive KRAB domain
227010pFUGW hUbC-Sin-dCas9-KRAB-T2A-GFPLentiviral Expression of dCas9-KRAB from S. iniae fused to the repressive KRAB domain
227011pFUGW hUbC-Spa-dCas9-KRAB-T2A-GFPLentiviral Expression of dCas9-KRAB from S. parasanguinis fused to the repressive KRAB domain
227012pFUGW hUbC-Sub-dCas9-KRAB-T2A-GFPLentiviral Expression of dCas9-KRAB from S. uberis fused to the repressive KRAB domain

Antibodies from Article