Characterization of diverse Cas9 orthologs for genome and epigenome editing.
Butterfield GL, Rohm D, Roberts A, Nethery MA, Rizzo AJ, Morone DJ, Garnier L, Iglesias N, Barrangou R, Gersbach CA
Proc Natl Acad Sci U S A. 2025 Mar 18;122(11):e2417674122. doi: 10.1073/pnas.2417674122. Epub 2025 Mar 12.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 227005 | pcDNA-Sga-Cas9 | Mammalian expression of Nuclease active S. gallolyticus Cas9 |
| 227006 | pcDNA-Sin-Cas9 | Mammalian expression of Nuclease active S. iniae Cas9 |
| 227007 | pcDNA-Spa-Cas9 | Mammalian expression of Nuclease active S. parasanguinis Cas9 |
| 227008 | pcDNA-Sub-Cas9 | Mammalian expression of Nuclease active S. uberis Cas9 |
| 227009 | pFUGW hUbC-Sga-dCas9-KRAB-T2A-GFP | Lentiviral Expression of dCas9-KRAB from S. gallolyticus fused to the repressive KRAB domain |
| 227010 | pFUGW hUbC-Sin-dCas9-KRAB-T2A-GFP | Lentiviral Expression of dCas9-KRAB from S. iniae fused to the repressive KRAB domain |
| 227011 | pFUGW hUbC-Spa-dCas9-KRAB-T2A-GFP | Lentiviral Expression of dCas9-KRAB from S. parasanguinis fused to the repressive KRAB domain |
| 227012 | pFUGW hUbC-Sub-dCas9-KRAB-T2A-GFP | Lentiviral Expression of dCas9-KRAB from S. uberis fused to the repressive KRAB domain |