Richard Baker Lab: Molecular Toolbox
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 228479 | MZB087 | "Empty" pLib vector (biGBac System) for easy DNA Assembly |
| 228480 | MZB116 | "Empty" C-terminal self-cleaving CPD-tag vector for rapid cloning/DNA Assembly via SwaI restriction enzyme site (bacterial expression) |
| 228481 | MZB117 | "Empty" C-terminal HRV-3C cleavable GST tag vector for rapid cloning/DNA Assembly via NheI restriction enzyme site (Bacterial Expression) |
| 228482 | MZB126 | "Empty" HA-secretion (membrane) vector for easy DNA Assembly (Mammalian cell Expression) |
| 228483 | MZB129 | "Empty" HSA-secretion vector for easy DNA Assembly (Mammalian cell Expression) |
| 228484 | MZB133 | "Empty" N-terminal TEV cleavable 6x His tag vector for rapid cloning/DNA Assembly via SmaI restriction site (Bacterial Expression) |
| 228485 | MZB134 | "Empty" N-terminal 6xHis-TEV-Halotag-HRV vector for easy DNA Assembly for pulldowns (Bacterial Expression) |
| 228486 | MZB161 | "Empty" vector for easy DNA Assembly (Mammalian cell Expression) |
| 228487 | MZB174 | 10xHis-SUMO (Smt3) fused, HRV-3C cleavable, GST-tag for rapid cloning/DNA assembly of pulldown targets via NotI restriction site for E. coli based protein expression |
| 228488 | MZB184 | Empty 10xHis-SUMO (Smt3) vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression |
| 228489 | MZB186 | Empty 10xHis-SUMO (Smt3) fused Avi-tag vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression |
| 228490 | MZB205 | Empty 10xHis-SUMO (Smt3) fused Avi-tag, HRV-3C cleavable vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression |
| 228491 | MZB223 | Empty C-terminal 8xHis tag vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression |
| 228492 | MZB209 | BirA encoded pACYC vector for co-expression with Avi-tagged constructs (Bacterial Expression) |
| 228493 | MZB206 | Bacterial expression of 10xHis-SUMO (Smt3) fused Avi-tag, HRV-3C cleavable MSP2N2 for biotinylated nanodiscs |
| 228494 | MZB211 | 10xHis-SUMO (Smt3) tagged Ssod7-fused Pfu Polymerase with fidelity enhancing mutation (E. coli based protein expression) |
| 228495 | pChillyBoys_gen1 | pACYCDuet-1 encoding Oleispira antarctica GroEL and GorES low temp chaperonins for enhanced low-temperature induction (E. coli based protein expression) |
| 228496 | pHyperTEV60 | 10xHis-SUMO (Smt3) tagged HyperTEV60 engineered protease (E. coli based protein expression) |
| 228498 | SUMO-PNGaseF | 10xHis-SUMO (Smt3) tagged PNGaseF (E. coli based protein expression) |