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Addgene

Richard Baker Lab: Molecular Toolbox

Unpublished

Plasmids from Article

ID Plasmid Purpose
228479MZB087"Empty" pLib vector (biGBac System) for easy DNA Assembly
228481MZB117"Empty" C-terminal HRV-3C cleavable GST tag vector for rapid cloning/DNA Assembly via NheI restriction enzyme site (Bacterial Expression)
228482MZB126"Empty" HA-secretion (membrane) vector for easy DNA Assembly (Mammalian cell Expression)
228483MZB129"Empty" HSA-secretion vector for easy DNA Assembly (Mammalian cell Expression)
228484MZB133"Empty" N-terminal TEV cleavable 6x His tag vector for rapid cloning/DNA Assembly via SmaI restriction site (Bacterial Expression)
228485MZB134"Empty" N-terminal 6xHis-TEV-Halotag-HRV vector for easy DNA Assembly for pulldowns (Bacterial Expression)
228494MZB21110xHis-SUMO (Smt3) tagged Ssod7-fused Pfu Polymerase with fidelity enhancing mutation (E. coli based protein expression)
228495pChillyBoys_gen1pACYCDuet-1 encoding Oleispira antarctica GroEL and GorES low temp chaperonins for enhanced low-temperature induction (E. coli based protein expression)
228498SUMO-PNGaseF10xHis-SUMO (Smt3) tagged PNGaseF (E. coli based protein expression)

Antibodies from Article