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RAD-TGTs: high-throughput measurement of cellular mechanotype via rupture and delivery of DNA tension probes.

Pawlak MR, Smiley AT, Ramirez MP, Kelly MD, Shamsan GA, Anderson SM, Smeester BA, Largaespada DA, Odde DJ, Gordon WR
Nat Commun. 2023 Apr 28;14(1):2468. doi: 10.1038/s41467-023-38157-6. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
226983WDVExpresses the HUH endonuclease WDV, used as a no ligand control for DNA tension sensor experiments
226985WDV-EchistatinExpresses the HUH endonuclease WDV fused at the C termini with Echistatin to target RGD binding integrins with DNA tension sensors

Antibodies from Article