Mechanistic Studies of a Skatole-Forming Glycyl Radical Enzyme Suggest Reaction Initiation via Hydrogen Atom Transfer.
Fu B, Nazemi A, Levin BJ, Yang Z, Kulik HJ, Balskus EP
J Am Chem Soc. 2022 Jun 29;144(25):11110-11119. doi: 10.1021/jacs.1c13580. Epub 2022 Jun 15.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 226967 | pET28a-OuIAD | Expressing Olsenella uli indoleacetate decarboxylase (IAD) in E. coli |
| 226968 | pET28a-OuIAD-AE | Expressing Olsenella uli indoleacetate decarboxylase activating enzyme (IAD-AE) in E. coli |
| 226969 | pET28a-OuIAD(G853A) | Expressing Olsenella uli indoleacetate decarboxylase (IAD) G853A variant in E. coli |
| 226970 | pET28a-OuIAD(C500S) | Expressing Olsenella uli indoleacetate decarboxylase (IAD) C500S variant in E. coli |
| 226971 | pET28a-OuIAD(H514A) | Expressing Olsenella uli indoleacetate decarboxylase (IAD) H514A variant in E. coli |
| 226972 | pET28a-OuIAD(H514E) | Expressing Olsenella uli indoleacetate decarboxylase (IAD) H514E variant in E. coli |
| 226974 | pET28a-OuIAD(R226E) | Expressing Olsenella uli indoleacetate decarboxylase (IAD) R226E variant in E. coli |
| 226975 | pET28a-OuIAD(R226M) | Expressing Olsenella uli indoleacetate decarboxylase (IAD) R226M variant in E. coli |
| 226976 | pET28a-OuIAD(R226K) | Expressing Olsenella uli indoleacetate decarboxylase (IAD) R226K variant in E. coli |
| 226977 | pET28a-OuIAD(L616E) | Expressing Olsenella uli indoleacetate decarboxylase (IAD) L616E variant in E. coli |
| 226978 | pET28a-OuIAD(F401A) | Expressing Olsenella uli indoleacetate decarboxylase (IAD) F401A variant in E. coli |
| 226979 | pET28a-OuIAD(W392F) | Expressing Olsenella uli indoleacetate decarboxylase (IAD) W392F variant in E. coli |
| 226980 | pET28a-OuIAD(W392A) | Expressing Olsenella uli indoleacetate decarboxylase (IAD) W392A variant in E. coli |
| 226981 | pET28a-CsHPAD_beta-gamma | Expressing Clostridium scatologenes hydroxyphenylacetate decarboxylase (HPAD) beta/gamma subunits in E. coli |
| 226982 | pET28a-CsHPAD-AE | Expressing Clostridium scatologenes hydroxyphenylacetate decarboxylase activating enzyme (HPAD-AE) in E. coli |